ArticleMolecular and cellular biology2012
Paf1 restricts Gcn4 occupancy and antisense transcription at the ARG1 promoter.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.Autophagy · 2020Article
- Yeast PAF1 complex counters the pol III accumulation and replication stress on the tRNA genes.Scientific reports · 2019Article
- PAF1 complex component Leo1 helps recruitProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Drosophila CG2469 Encodes a Homolog of Human CTR9 and Is Essential for Development.G3 (Bethesda, Md.) · 2016Article
- Interaction of Gcn4 with target gene chromatin is modulated by proteasome function.Molecular biology of the cell · 2016Article
- Sense and antisense transcription are associated with distinct chromatin architectures across genes.Nucleic acids research · 2015Article
- Heavy transcription of yeast genes correlates with differential loss of histone H2B relative to H4 and queued RNA polymerases.Nucleic acids research · 2014Article
- IL-1β-specific recruitment of GCN5 histone acetyltransferase induces the release of PAF1 from chromatin for the de-repression of inflammatory response genes.Nucleic acids research · 2013Article
- The many roles of the conserved eukaryotic Paf1 complex in regulating transcription, histone modifications, and disease states.Biochimica et biophysica acta · 2013Article
- Effects of the Paf1 complex and histone modifications on snoRNA 3'-end formation reveal broad and locus-specific regulation.Molecular and cellular biology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The conserved Paf1 complex negatively regulates the expression of numerous genes, yet the mechanisms by which it represses gene expression are not well understood. In this study, we use the ARG1 gene as a model to investigate the repressive functions of the Paf1 complex in Saccharomyces cerevisiae. Our results indicate that Paf1 mediates repression of the ARG1 gene independently of the gene-specific repressor, ArgR/Mcm1. Rather, by promoting histone H2B lysine 123 ubiquitylation, Paf1 represses the ARG1 gene by negatively affecting Gcn4 occupancy at the promoter. Consistent with this observation, Gcn5 and its acetylation sites on histone H3 are required for full ARG1 derepression in paf1Δ cells, and the repressive effect of Paf1 is largely maintained when the ARG1 promoter directs transcription of a heterologous coding region. Derepression of the ARG1 gene in paf1Δ cells is accompanied by small changes in nucleosome occupancy, although these changes are subtle in comparison to those that accompany gene activation through amino acid starvation. Additionally, conditions that stimulate ARG1 transcription, including PAF1 deletion, lead to increased antisense transcription across the ARG1 promoter. This promoter-associated antisense transcription positively correlates with ARG1 sense transcription. Finally, our results indicate that Paf1 represses other genes through mechanisms similar to those used at the ARG1 gene.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.