ReviewMethods (San Diego, Calif.)2012
Vaccine applications of flow cytometry.
Review in Methods (San Diego, Calif.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Baseline host determinants of robust human HIV-1 vaccine-induced immune responses: A meta-analysis of 26 vaccine regimens.EBioMedicine · 2022Pooled it
- Trial
- A flow cytometry-based workflow for detection and quantification of anti-plasmodial antibodies in vaccinated and naturally exposed individuals.Malaria journal · 2012Trial
- Comprehensive Analytical Characterization of mRNA Influenza Vaccine Under Stress Conditions: Implications for Stability and Quality Control.Pharmaceutical research · 2026Article
- An at-home blood collection device for remote immune monitoring by high-parameter flow cytometry.JCI insight · 2026Article
- Mini-review on the therapeutic vaccines targeting chronic infectious diseases: Evaluation system of therapeutic vaccines targeting HPV and EBV-related cancers.Human vaccines & immunotherapeutics · 2025Review
- Generalized linear modeling of flow cytometry data to analyze immune responses in tuberculosis vaccine research.NPJ systems biology and applications · 2025Article
- The Application of Single-Cell Technologies for Vaccine Development Against Viral Infections.Vaccines · 2025Review
- Imaging flow cytometry reveals the mechanism of equine arteritis virus entry and internalization.Scientific reports · 2025Article
- Evaluation of two commercial IBR marker vaccines againstFrontiers in veterinary science · 2025Article
- Applications of Flow Cytometry in Drug Discovery and Translational Research.International journal of molecular sciences · 2024Review
- Evaluation of Cellular Immune Response to Adeno-Associated Virus-Based Gene Therapy.The AAPS journal · 2023Review
- Immune monitoring of SARS-CoV-2-specific T cell and B cell responses in patients with multiple sclerosis treated with ocrelizumab.Frontiers in immunology · 2023Article
- Mechanisms of Particles in Sensitization, Effector Function and Therapy of Allergic Disease.Frontiers in immunology · 2020Review
- The role of systems biology approaches in determining molecular signatures for the development of more effective vaccines.Expert review of vaccines · 2019Review
- Characterization of T-cell immune responses in clinical trials of the candidate RTS,S malaria vaccine.Human vaccines & immunotherapeutics · 2018Review
- High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometry.Virology · 2017Article
- Immunity in the spleen and blood of mice immunized with irradiated Toxoplasma gondii tachyzoites.Medical microbiology and immunology · 2016Article
- Minor Antigen Vaccine-Sensitized DLI: In Vitro Responses Do Not Predict In Vivo Effects.Transplantation direct · 2016Article
- Flow Cytometric Analysis of Protective T-Cell Response Against Pulmonary Coccidioides Infection.Methods in molecular biology (Clifton, N.J.) · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
Highly effective vaccines have yet to be identified for many widespread infectious diseases including HIV, tuberculosis and malaria. Many vaccine candidates for these diseases have been designed to induce both cellular and humoral immunity, and measurement of the induced cellular immune response and antibody response is critical for monitoring immunogenicity. The flow cytometric intracellular cytokine staining assay is one of the primary assays for enumerating vaccine-induced T cells in vaccine clinical trials. The assay is flexible, allowing for measurement of various cytokines or functions and phenotyping markers, and the assay can be validated. Changes in other cell types such as innate immune cells are monitored by flow cytometric phenotyping assays. Cell sorting of vaccine-induced T cells and B cells is used to allow genomic and transcriptional analysis of these cells. Thus, flow cytometric methods are commonly used in trials testing the next generation of vaccines.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.