ArticleThe Journal of physiology2012
Cotransport of water by Na⁺-K⁺-2Cl⁻ cotransporters expressed in Xenopus oocytes: NKCC1 versus NKCC2.
Article in The Journal of physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 107 citations in OpenAlex.
- Exploring and translating novel approaches targeting the choroid plexus for the treatment of hydrocephalus: a literature review.Fluids and barriers of the CNS · 2026Review
- Serendipitous compound action potential oscillations reveal glycolytic astrocyte and oxidative axon interstitial KExperimental physiology · 2026Article
- The NaAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Diuretics: a review of the pharmacology and effects on glucose homeostasis.Frontiers in pharmacology · 2025Review
- Intraventricular haemorrhage in premature infants: the role of immature neuronal salt and water transport.Brain : a journal of neurology · 2024Article
- Aqp4a and Trpv4 mediate regulatory cell volume increase for swimming maintenance of marine fish spermatozoa.Cellular and molecular life sciences : CMLS · 2024Article
- Circadian Mechanisms in Brain Fluid Biology.Circulation research · 2024Review
- Cation Chloride Cotransporter NKCC1 Operates through a Rocking-Bundle Mechanism.Journal of the American Chemical Society · 2024Article
- Role of NKCC1 and KCC2 during hypoxia-induced neuronal swelling in the neonatal neocortex.Neurobiology of disease · 2023Article
- The choroid plexus: a missing link in our understanding of brain development and function.Physiological reviews · 2023Review
- Non-Aquaporin Water Channels.Advances in experimental medicine and biology · 2023Article
- Honey, I shrunk the extracellular space: Measurements and mechanisms of astrocyte swelling.Glia · 2022Review
- Membrane transporters control cerebrospinal fluid formation independently of conventional osmosis to modulate intracranial pressure.Fluids and barriers of the CNS · 2022Article
- Calcium-dependent protein kinase 16 phosphorylates and activates the aquaporin PIP2;2 to regulate reversible flower opening in Gentiana scabra.The Plant cell · 2022Article
- Cerebrospinal fluid production by the choroid plexus: a century of barrier research revisited.Fluids and barriers of the CNS · 2022Review
- Molecular mechanisms of brain water transport.Nature reviews. Neuroscience · 2021Review
- The structural basis of function and regulation of neuronal cotransporters NKCC1 and KCC2.Communications biology · 2021Article
- Choroid plexus NKCC1 mediates cerebrospinal fluid clearance during mouse early postnatal development.Nature communications · 2021Article
- From Pinocytosis to Methuosis-Fluid Consumption as a Risk Factor for Cell Death.Frontiers in cell and developmental biology · 2021Review
- TRPing on Cell Swelling - TRPV4 Senses It.Frontiers in immunology · 2021Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NKCC1 and NKCC2 isoforms of the mammalian Na⁺–K⁺–2Cl⁻ cotransporter were expressed in Xenopus oocytes and the relation between external ion concentration and water fluxes determined.Water fluxes were determined from changes in the oocytes volume and ion fluxes from 86Rb+ uptake. Isotonic increases in external K⁺ concentration elicited abrupt inward water fluxes in NKCC1; the K⁺ dependence obeyed one-site kinetics with a K₀.₅ of 7.5 mM. The water fluxes were blocked by bumetanide, had steep temperature dependence and could proceed uphill against an osmotic gradient of 20 mosmol l⁻¹. A comparison between ion and water fluxes indicates that 460 water molecules are cotransported for each turnover of the protein. In contrast, NKCC2 did not support water fluxes.Water transport in NKCC1 induced by increases in the external osmolarity had high activation energy and was blocked by bumetanide. The osmotic effects of NaCl were smaller than those of urea and mannitol. This supports the notion of interaction between ions and water in NKCC1 and allows for an estimate of around 600 water molecules transported per turnover of the protein. Osmotic gradients did not induce water transport in NKCC2. We conclude that NKCC1 plays a direct role for water balance in most cell types, while NKCC2 fulfils its role in the kidney of transporting ions but not water. The different behaviour of NKCC1 and NKCC2 is discussed on the basis of recent molecular models based on studies of structural and molecular dynamics.
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