ArticleThe Journal of biological chemistry2012
The Drosophila juvenile hormone receptor candidates methoprene-tolerant (MET) and germ cell-expressed (GCE) utilize a conserved LIXXL motif to bind the FTZ-F1 nuclear receptor.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 51 citations in OpenAlex.
- Characterization of the Crustacean Methyl Farnesoate Transcriptional Signaling Genes.International journal of molecular sciences · 2026Article
- Article
- The Juvenile-Hormone-Responsive FactorBiomolecules · 2023Article
- Article
- Juvenile hormone receptor Methoprene tolerant: Functions and applications.Vitamins and hormones · 2023Article
- Interaction patterns of methoprene-tolerant and germ cell-expressedFrontiers in molecular biosciences · 2023Article
- Dissecting the Isoform-Specific Roles of FTZ-F1 in the Larval-Larval and Larval-Pupal Ecdyses inInsects · 2022Article
- Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83.Frontiers in physiology · 2022Article
- Article
- Juvenile Hormone Studies inFrontiers in physiology · 2021Review
- The intrinsically disordered region of GCE protein adopts a more fixed structure by interacting with the LBD of the nuclear receptor FTZ-F1.Cell communication and signaling : CCS · 2020Article
- Juvenile hormone regulates the shift from migrants to residents in adult oriental armyworm, Mythimna separata.Scientific reports · 2020Article
- bHLH-PAS Proteins: Their Structure and Intrinsic Disorder.International journal of molecular sciences · 2019Review
- Genome-Wide Screening and Functional Analysis Reveal That the Specific microRNA nlu-miR-173 Regulates Molting by Targeting Ftz-F1 inFrontiers in physiology · 2018Article
- Article
- Distinct Roles of Met and Interacting Proteins on the Expressions ofFrontiers in physiology · 2017Article
- Sequence Conservation and Sexually Dimorphic Expression of the Ftz-F1 Gene in the Crustacean Daphnia magna.PloS one · 2016Article
- Mapping of the Sequences Directing Localization of the Drosophila Germ Cell-Expressed Protein (GCE).PloS one · 2015Article
- Juvenile hormone-receptor complex acts on mcm4 and mcm7 to promote polyploidy and vitellogenesis in the migratory locust.PLoS genetics · 2014Article
- Population genomics of the honey bee reveals strong signatures of positive selection on worker traits.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Juvenile hormone (JH) has been implicated in many developmental processes in holometabolous insects, but its mechanism of signaling remains controversial. We previously found that in Drosophila Schneider 2 cells, the nuclear receptor FTZ-F1 is required for activation of the E75A gene by JH. Here, we utilized insect two-hybrid assays to show that FTZ-F1 interacts with two JH receptor candidates, the bHLH-PAS paralogs MET and GCE, in a JH-dependent manner. These interactions are severely reduced when helix 12 of the FTZ-F1 activation function 2 (AF2) is removed, implicating AF2 as an interacting site. Through homology modeling, we found that MET and GCE possess a C-terminal α-helix featuring a conserved motif LIXXL that represents a novel nuclear receptor (NR) box. Docking simulations supported by two-hybrid experiments revealed that FTZ-F1·MET and FTZ-F1·GCE heterodimer formation involves a typical NR box-AF2 interaction but does not require the canonical charge clamp residues of FTZ-F1 and relies primarily on hydrophobic contacts, including a unique interaction with helix 4. Moreover, we identified paralog-specific features, including a secondary interaction site found only in MET. Our findings suggest that a novel NR box enables MET and GCE to interact JH-dependently with the AF2 of FTZ-F1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.