ArticlePlasmid2012
In situ monitoring of IncF plasmid transfer on semi-solid agar surfaces reveals a limited invasion of plasmids in recipient colonies.
Article in Plasmid, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 47 citations in OpenAlex.
- Microbe-plant interactions ofEcoSal Plus · 2025Review
- Biofilm architecture determines the dissemination of conjugative plasmids.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Encounter rates and engagement times limit the transmission of conjugative plasmids.PLoS genetics · 2025Article
- The F plasmid conjutome: the repertoire ofmSphere · 2024Article
- Phage predation accelerates the spread of plasmid-encoded antibiotic resistance.Nature communications · 2024Article
- Dissemination of IncI plasmid encodingAntimicrobial agents and chemotherapy · 2023Article
- CRISPR-Cas-Based Antimicrobials: Design, Challenges, and Bacterial Mechanisms of Resistance.ACS infectious diseases · 2023Review
- Timing of antibiotic administration determines the spread of plasmid-encoded antibiotic resistance during microbial range expansion.Nature communications · 2023Article
- In Situ Monitoring and Quantitative Determination of R27 Plasmid Conjugation.Life (Basel, Switzerland) · 2022Article
- Genome-Wide Association Study Reveals Host Factors Affecting Conjugation inMicroorganisms · 2022Article
- Article
- Plasmid Transfer by Conjugation in Gram-Negative Bacteria: From the Cellular to the Community Level.Genes · 2020Review
- The Less Expensive Choice: Bacterial Strategies to Achieve Successful and Sustainable Reciprocal Interactions.Frontiers in microbiology · 2020Review
- Cooperative Function of TraJ and ArcA in Regulating the F PlasmidJournal of bacteriology · 2019Article
- Plasmid transfer in biofilms: a perspective on limitations and opportunities.NPJ biofilms and microbiomes · 2016Article
- Genetic drift suppresses bacterial conjugation in spatially structured populations.Biophysical journal · 2014Article
- Social behavior and decision making in bacterial conjugation.Frontiers in cellular and infection microbiology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Most natural conjugative IncF plasmids encode a fertility inhibition system that represses transfer gene expression in the majority of plasmid-carrying cells. The successful spread of these plasmids in clinically relevant bacteria has been suggested to be supported by a transitory derepression of transfer gene expression in newly formed transconjugants. In this study, we aimed to monitor the extent of transitory derepression during agar surface matings in situ by comparing plasmid spread of the IncF plasmid R1 and its derepressed mutant R1drd19 at low initial cell densities. A zygotic induction strategy was used to visualize the spatial distribution of fluorescent transconjugants within the heterogeneous environment. Epifluorescence and confocal microscopy revealed different transfer patterns for both plasmids, however, spread beyond the first five recipient cell layers adjacent to the donor cells was not observed. Similar results were observed for other prototypical conjugative plasmids. These results cannot rule out that transitory derepression contributes to the limited R1 plasmid invasion, but other factors like nutrient availability or spatial structure seem to limit plasmid spread.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.