ArticleExperimental cell research2012
RIM, Munc13, and Rab3A interplay in acrosomal exocytosis.
Article in Experimental cell research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Lipid remodeling in acrosome exocytosis: unraveling key players in the human sperm.Frontiers in cell and developmental biology · 2024Review
- Vesicle trafficking and vesicle fusion: mechanisms, biological functions, and their implications for potential disease therapy.Molecular biomedicine · 2022Review
- Identification of a Signature for Predicting Prognosis and Immunotherapy Response in Patients with Glioma.Journal of oncology · 2022Article
- Synaptic Secretion and Beyond: Targeting Synapse and Neurotransmitters to Treat Neurodegenerative Diseases.Oxidative medicine and cellular longevity · 2022Review
- VAMPs sensitive to tetanus toxin are required for cortical granule exocytosis in mouse oocytes.Experimental cell research · 2021Article
- The Rab3A-22A Chimera Prevents Sperm Exocytosis by Stabilizing Open Fusion Pores.The Journal of biological chemistry · 2016Article
- Rab3A, a possible marker of cortical granules, participates in cortical granule exocytosis in mouse eggs.Experimental cell research · 2016Article
- ADP ribosylation factor 6 (ARF6) promotes acrosomal exocytosis by modulating lipid turnover and Rab3A activation.The Journal of biological chemistry · 2015Article
- Article
- Functional distribution of synapsin I in human sperm.FEBS open bio · 2015Article
- Epac, Rap and Rab3 act in concert to mobilize calcium from sperm's acrosome during exocytosis.Cell communication and signaling : CCS · 2014Article
- NAADP and the two-pore channel protein 1 participate in the acrosome reaction in mammalian spermatozoa.Molecular biology of the cell · 2014Article
- The cortical acto-Myosin network: from diffusion barrier to functional gateway in the transport of neurosecretory vesicles to the plasma membrane.Frontiers in endocrinology · 2013Review
- Liprin α3: a putative estrogen regulated acrosomal protein.Histochemistry and cell biology · 2013Article
- K+ and Cl- channels and transporters in sperm function.Current topics in developmental biology · 2013Review
- MARCKS protein is phosphorylated and regulates calcium mobilization during human acrosomal exocytosis.PloS one · 2013Article
- Article
- Mouse sperm membrane potential hyperpolarization is necessary and sufficient to prepare sperm for the acrosome reaction.The Journal of biological chemistry · 2012Article
- Munc18-1 controls SNARE protein complex assembly during human sperm acrosomal exocytosis.The Journal of biological chemistry · 2012Article
- Putting things in place for fertilization: discovering roles for importin proteins in cell fate and spermatogenesis.Asian journal of andrologyReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Exocytosis is a highly regulated, multistage process consisting of multiple functionally definable stages, including recruitment, targeting, tethering, priming, and docking of secretory vesicles with the plasma membrane, followed by calcium-triggered membrane fusion. The acrosome reaction of spermatozoa is a complex, calcium-dependent regulated exocytosis. Fusion at multiple sites between the outer acrosomal membrane and the cell membrane causes the release of the acrosomal contents and the loss of the membranes surrounding the acrosome. Not much is known about the molecules that mediate membrane docking in this particular fusion model. In neurons, the formation of the ternary RIM/Munc13/Rab3A complex has been suggested as a critical component of synaptic vesicles docking. Previously, we demonstrated that Rab3A localizes to the acrosomal region in human sperm, stimulates acrosomal exocytosis, and participates in an early stage during membrane fusion. Here, we report that RIM and Munc13 are also present in human sperm and localize to the acrosomal region. Like Rab3A, RIM and Munc13 participate in a prefusion step before the efflux of intra-acrosomal calcium. By means of a functional assay using antibodies and recombinant proteins, we show that RIM, Munc13 and Rab3A interplay during acrosomal exocytosis. Finally, we report by electron transmission microscopy that sequestering RIM and Rab3A alters the docking of the acrosomal membrane to the plasma membrane during calcium-activated acrosomal exocytosis. Our results suggest that the RIM/Munc13/Rab3 A complex participates in acrosomal exocytosis and that RIM and Rab3A have central roles in membrane docking.
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