ArticleBMC genomics2012
Quantification and accurate normalisation of small RNAs through new custom RT-qPCR arrays demonstrates Salmonella-induced microRNAs in human monocytes.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Human cells contain myriad excised linear intron RNAs with links to gene regulation and potential utility as biomarkers.PLoS genetics · 2024Article
- Systematic analysis of different degrees of haemolysis on miRNA levels in serum and serum-derived extracellular vesicles from dogs.BMC veterinary research · 2022Article
- The glycosyltransferase ST3GAL2 is regulated by miR-615-3p in the intestinal tract of Campylobacter jejuni infected mice.Gut pathogens · 2021Article
- Key Macrophage Responses to Infection WithFrontiers in immunology · 2021Article
- Deregulation of miR-27a may contribute to canine fibroblast activation after coculture with a mast cell tumour cell line.FEBS open bio · 2020Article
- Characterization of miRNAs in Extracellular Vesicles Released From Atlantic Salmon Monocyte-Like and Macrophage-Like Cells.Frontiers in immunology · 2020Article
- Long Noncoding RNAs in Host-Pathogen Interactions.Trends in immunology · 2019Review
- Multifaceted Roles of microRNAs in Host-Bacterial Pathogen Interaction.Microbiology spectrum · 2019Review
- MicroRNAs as Molecular Switches in Macrophage Activation.Frontiers in immunology · 2019Review
- miR-181a-5p Regulates TNF-α and miR-21a-5p Influences Gualynate-Binding Protein 5 and IL-10 Expression in Macrophages Affecting Host Control ofFrontiers in immunology · 2018Article
- Cecal MicroRNAome response to Salmonella enterica serovar Enteritidis infection in White Leghorn Layer.BMC genomics · 2017Article
- TFF3-dependent resistance of human colorectal adenocarcinoma cells HT-29/B6 to apoptosis is mediated by miR-491-5p regulation of lncRNA PRINS.Cell death discovery · 2017Article
- A Long Journey Ahead: Long Non-coding RNAs in Bacterial Infections.Frontiers in cellular and infection microbiology · 2017Review
- MicroRNA Response of Primary Human Macrophages to Arcobacter Butzleri Infection.European journal of microbiology & immunology · 2016Article
- Down regulated lncRNA MEG3 eliminates mycobacteria in macrophages via autophagy.Scientific reports · 2016Article
- MicroRNA Cargo of Extracellular Vesicles from Alcohol-exposed Monocytes Signals Naive Monocytes to Differentiate into M2 Macrophages.The Journal of biological chemistry · 2016Article
- Small Non-Coding RNAs: New Insights in Modulation of Host Immune Response by Intracellular Bacterial Pathogens.Frontiers in immunology · 2016Review
- Modulation of Host miRNAs by Intracellular Bacterial Pathogens.Frontiers in cellular and infection microbiology · 2016Review
- Mycobacterium bovis BCG Interferes with miR-3619-5p Control of Cathepsin S in the Process of Autophagy.Frontiers in cellular and infection microbiology · 2016Article
- Alcohol-induced miR-27a regulates differentiation and M2 macrophage polarization of normal human monocytes.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSmall interfering and non-coding RNAs regulate gene expression across all kingdoms of life. MicroRNAs constitute an important group of metazoan small RNAs regulating development but also disease. Accordingly, in functional genomics microRNA expression analysis sheds more and more light on the dynamic regulation of gene expression in various cellular processes.
resultsWe have developed custom RT-qPCR arrays allowing for accurate quantification of 31 small RNAs in triplicate using a 96 well format. In parallel, we provide accurate normalisation of microRNA expression data based on the quantification of 5 reference snRNAs. We have successfully employed such arrays to study microRNA regulation during human monocyte differentiation as well as Salmonella infection. Besides well-known protagonists such as miR-146 or miR-155, we identified the up-regulation of miR-21, miR-222, miR-23b, miR-24, miR-27a as well as miR-29 upon monocyte differentiation or infection, respectively.
conclusionsThe provided protocol for RT-qPCR arrays enables straight-forward microRNA expression analysis. It is fully automatable, compliant with the MIQE guidelines and can be completed in only 1 day. The application of these arrays revealed microRNAs that may mediate monocyte host defence mechanisms by regulating the TGF-β signalling upon Salmonella infection. The introduced arrays are furthermore suited for customised quantification of any class of small non-coding RNAs as exemplified by snRNAs and thus provide a versatile tool for ubiquitous applications.
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