ArticleJournal of bacteriology2012
Further unraveling the regulatory twist by elucidating metabolic coinducer-mediated CbbR-cbbI promoter interactions in Rhodopseudomonas palustris CGA010.
Article in Journal of bacteriology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Effect of COFrontiers in microbiology · 2019Article
- An Adaptive Mutation in Enterococcus faecium LiaR Associated with Antimicrobial Peptide Resistance Mimics Phosphorylation and Stabilizes LiaR in an Activated State.Journal of molecular biology · 2016Article
- Phosphoribulokinase mediates nitrogenase-induced carbon dioxide fixation gene repression in Rhodobacter sphaeroides.Microbiology (Reading, England) · 2015Article
- CbbR, the Master Regulator for Microbial Carbon Dioxide Fixation.Journal of bacteriology · 2015Review
- The effect of CbbR-binding affinity to the upstream of cbbF and cfxB on the metabolic effector in Rhodobacter sphaeroides.Current microbiology · 2015Article
- A variable DNA recognition site organization establishes the LiaR-mediated cell envelope stress response of enterococci to daptomycin.Nucleic acids research · 2015Article
- Identification and characterization of VpsR and VpsT binding sites in Vibrio cholerae.Journal of bacteriology · 2015Article
- Evolutionary expansion of a regulatory network by counter-silencing.Nature communications · 2014Article
- Amino acid residues of RegA important for interactions with the CbbR-DNA complex of Rhodobacter sphaeroides.Journal of bacteriology · 2014Article
- Regulatory twist and synergistic role of metabolic coinducer- and response regulator-mediated CbbR-cbbI interactions in Rhodopseudomonas palustris CGA010.Journal of bacteriology · 2013Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cbb(I) region of Rhodopseudomonas palustris (Rp. palustris) contains the cbbLS genes encoding form I ribulose-1,5-bisphosphate (RuBP) carboxylase oxygenase (RubisCO) along with a divergently transcribed regulator gene, cbbR. Juxtaposed between cbbR and cbbLS are the cbbRRS genes, encoding an unusual three-protein two-component (CbbRRS) system that modulates the ability of CbbR to influence cbbLS expression. The nature of the metabolic signals that Rp. palustris CbbR perceives to regulate cbbLS transcription is not known. Thus, in this study, the CbbR binding region was first mapped within the cbbLS promoter by the use of gel mobility shift assays and DNase I footprinting. In addition, potential metabolic coinducers (metabolites) were tested for their ability to alter the cbbLS promoter binding properties of CbbR. Gel mobility shift assays and surface plasmon resonance analyses together indicated that biosynthetic intermediates such as RuBP, ATP, fructose 1,6-bisphosphate, and NADPH enhanced DNA binding by CbbR. These coinducers did not yield identical CbbR-dependent DNase I footprints, indicating that the coinducers caused significant changes in DNA structure. These in vitro studies suggest that cellular signals such as fluctuating metabolite concentrations are perceived by and transduced to the cbbLS promoter via the master regulator CbbR.
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