ArticleArteriosclerosis, thrombosis, and vascular biology2012
Dynamic immune cell accumulation during flow-induced atherogenesis in mouse carotid artery: an expanded flow cytometry method.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 43 citations in OpenAlex.
- Identification of unannotated microproteins involved in endothelial cell homeostasis, dysfunction, and vascular disease.Cardiovascular research · 2026Article
- Endothelial Elavl1 Is Required for CD8 T-Cell Persistence in Atherosclerosis.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- The GRK2/AP-1 Signaling Axis Mediates Vascular Endothelial Dysfunction and Atherosclerosis Induced by Oscillatory Low Shear Stress.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Endothelial Immunosuppression in Atherosclerosis : Translational Control by Elavl1/HuR.bioRxiv : the preprint server for biology · 2024Article
- Cellular mechanisms of oligoclonal vascular smooth muscle cell expansion in cardiovascular disease.Cardiovascular research · 2023Article
- Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development.Journal of cardiovascular translational research · 2023Article
- Article
- Stable flow-induced expression of KLK10 inhibits endothelial inflammation and atherosclerosis.eLife · 2022Article
- Sox13 is a novel flow-sensitive transcription factor that prevents inflammation by repressing chemokine expression in endothelial cells.Frontiers in cardiovascular medicine · 2022Article
- Atorvastatin and blood flow regulate expression of distinctive sets of genes in mouse carotid artery endothelium.Current topics in membranes · 2021Article
- Emerging Roles for Neuropilin-2 in Cardiovascular Disease.International journal of molecular sciences · 2020Review
- Carotid Disease and Ageing: A Literature Review on the Pathogenesis of Vascular Senescence in Older Subjects.Current gerontology and geriatrics research · 2020Review
- Direct CD137 costimulation of CD8 T cells promotes retention and innate-like function within nascent atherogenic foci.American journal of physiology. Heart and circulatory physiology · 2019Article
- Smooth Muscle Cells Contribute the Majority of Foam Cells in ApoE (Apolipoprotein E)-Deficient Mouse Atherosclerosis.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Knockout rat models mimicking human atherosclerosis created by Cpf1-mediated gene targeting.Scientific reports · 2019Article
- Differential effects of short chain fatty acids on endothelial Nlrp3 inflammasome activation and neointima formation: Antioxidant action of butyrate.Redox biology · 2018Article
- Atherosclerosis is exacerbated by chitinase-3-like-1 in amyloid precursor protein transgenic mice.Theranostics · 2018Article
- Endothelial Mechanotransduction, Redox Signaling and the Regulation of Vascular Inflammatory Pathways.Frontiers in physiology · 2018Review
- Microbiota control acute arterial inflammation and neointimal hyperplasia development after arterial injury.PloS one · 2018Article
- Accelerated atherosclerosis development in C57Bl6 mice by overexpressing AAV-mediated PCSK9 and partial carotid ligation.Laboratory investigation; a journal of technical methods and pathology · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveInflammation plays a central role in atherosclerosis. However, the detailed changes in the composition and quantity of leukocytes in the arterial wall during atherogenesis are not fully understood in part because of the lack of suitable methods and animal models. METHODS AND
resultsWe developed a 10-fluorochrome, 13-parameter flow cytometry method to quantitate 7 major leukocyte subsets in a single digested arterial wall sample. Apolipoprotein E-deficient mice underwent left carotid artery (LCA) partial ligation and were fed a high-fat diet for 4 to 28 days. Monocyte/macrophages, dendritic cells, granulocytes, natural killer cells, and CD4 T cells significantly infiltrated the LCA as early as 4 days. Monocyte/macrophages and dendritic cells decreased between 7 and 14 days, whereas T-cell numbers remained steady. Leukocyte numbers peaked at 7 days, preceding atheroma formation at 14 days. B cells entered LCA by 14 days. Control right carotid and sham-ligated LCAs showed no significant infiltrates. Polymerase chain reaction and ELISA arrays showed that expression of proinflammatory cytokines and chemokines peaked at 7 and 14 days postligation, respectively.
conclusionThis is the first quantitative description of leukocyte number and composition over the life span of murine atherosclerosis. These results show that disturbed flow induces rapid and dynamic leukocyte accumulation in the arterial wall during the initiation and progression of atherosclerosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.