ArticleThe Plant cell2012
Suppression of Arabidopsis ARGONAUTE1-mediated slicing, transgene-induced RNA silencing, and DNA methylation by distinct domains of the Cucumber mosaic virus 2b protein.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
110 citing papers in PubMed, 204 citations in OpenAlex.
- Article
- Antiviral RNAi defective 4 (AVI4) encodes a lysophospholipid acyltransferase coopting with AVI2 and ATG8a to promote antiviral immunity in Arabidopsis.Plant communications · 2026Article
- Article
- Cucumber Mosaic Virus Promotes Sphingolipid Biosynthesis to Alleviate Host Unfolded Protein Response (UPR)-Mediated Immunity.Molecular plant pathology · 2026Article
- An integrated green control strategy for plant viral co-infections: from multivalent vaccine construction to synergistically modified nanodelivery systems.Journal of nanobiotechnology · 2026Article
- The Tomato Transcription Factor RAV Affects the Systemic Infection of TYLCV by Interacting With V2.Molecular plant pathology · 2026Article
- A mutually antagonistic mechanism mediated by RNA mNature communications · 2025Article
- Diverse RNA viral effectors converge on facilitation of AGO4 degradation to promote infection.Nucleic acids research · 2025Article
- Truncated CMV2bPlant methods · 2025Article
- The pathogen effector BcSSP2 suppresses the NPC phase separation to facilitate Botrytis cinerea infection.Nature communications · 2025Article
- Epigenetics in the modern era of crop improvements.Science China. Life sciences · 2025Review
- Potato Type I Protease Inhibitor Mediates Host Defence Against Potato Virus X Infection by Interacting With a Viral RNA Silencing Suppressor.Molecular plant pathology · 2025Article
- Moss-pathogen interactions: a review of the current status and future opportunities.Frontiers in genetics · 2025Review
- Advances in understanding the interaction betweenPlant signaling & behavior · 2024Review
- Viral coat proteins decrease the gene silencing activity of cognate and heterologous viral suppressors.Scientific reports · 2024Article
- Article
- Article
- Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC).Plant methods · 2024Article
- The trans-kingdom communication of noncoding RNAs in plant-environment interactions.The plant genome · 2023Review
- Review
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unique among the known plant and animal viral suppressors of RNA silencing, the 2b protein interacts directly with both small interfering RNA (siRNA) and ARGONAUTE1 (AGO1) and AGO4 proteins and is targeted to the nucleolus. However, it is largely unknown which regions of the 111-residue 2b protein determine these biochemical properties and how they contribute to its diverse silencing suppressor activities. Here, we identified a functional nucleolar localization signal encoded within the 61-amino acid N-terminal double-stranded RNA (dsRNA) binding domain (dsRBD) that exhibited high affinity for short and long dsRNA. However, physical interaction of 2b with AGOs required an essential 33-residue region C-terminal to the dsRBD and was sufficient to inhibit the in vitro AGO1 Slicer activity independently of its dsRNA binding activities. Furthermore, the direct 2b-AGO interaction was not essential for the 2b suppression of posttranscriptional gene silencing (PTGS) and RNA-directed DNA methylation (RdDM) in vivo. Lastly, we found that the 2b-AGO interactions in vivo also required the nucleolar targeting of 2b and had the potential to redistribute both the 2b and AGO proteins in nucleus. These findings together suggest that 2b may suppress PTGS and RdDM in vivo by binding and sequestering siRNA and the long dsRNA precursor in a process that is facilitated by its interactions with AGOs in the nucleolus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.