ArticleThe Plant cell2012
The rice pentatricopeptide repeat protein RF5 restores fertility in Hong-Lian cytoplasmic male-sterile lines via a complex with the glycine-rich protein GRP162.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 161 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
161 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A Systematic Review and Developmental Perspective on Origin of CMS Genes in Crops.International journal of molecular sciences · 2024Pooled it
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- Mitochondrial-Nuclear Interactions, Co-Transcription, and Adaptive Evolution in Cytoplasmic Male Sterility.Plant, cell & environment · 2026Review
- Pentatricopeptide repeat proteins in crops: Advances in functional mechanisms and breeding applications.Journal of integrative plant biology · 2026Review
- Novel cytoplasmic male sterile line development and stage-specific metabolomic profiling in rice (Oryza sativa L.) for advancing hybrid breeding technology.BMC plant biology · 2026Article
- Non-functional allele ofPlant biotechnology (Tokyo, Japan) · 2026Article
- A PPR gene cluster from Solanum aethiopicum underlies fertility restoration in cytoplasmic male-sterile eggplant (S. melongena L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Unraveling Advances in Rice Male Sterility Systems: From Genetic Basis to Hybrid Breeding Innovation.Plants (Basel, Switzerland) · 2026Review
- Genetic Resources of Cereal and Oilseed Crops for Heterotic Hybrid Breeding.Plants (Basel, Switzerland) · 2025Review
- Selection of reference genes for normalization of mitochondrial gene expression by qRT-PCR in different potato tissues and during anther development.BMC plant biology · 2025Article
- Emerging trends in transgene-free crop development: insights into genome editing and its regulatory overview.Plant molecular biology · 2025Review
- Enhancing drought resilience in crops: mechanistic approaches in the face of climate challenge.Plant molecular biology · 2025Review
- Two nuclear loci Rf-m1 and Rf-m2 interplay to determine the fertility restoring efficacy of ZD-type CMS soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
- Molecular and physiological basis of heterosis in hybrid rice performance.Molecular breeding : new strategies in plant improvement · 2025Review
- Gibberellin signalling mediates nucleocytoplasmic trafficking of Sucrose Synthase 1 to regulate the drought tolerance in rice.Plant biotechnology journal · 2025Article
- The Regulatory Roles of RNA-Binding Proteins in Plant Salt Stress Response.Plants (Basel, Switzerland) · 2025Review
- Identification of a gene coding for a pentatricopeptide repeat protein as a candidate responsible for theMolecular breeding : new strategies in plant improvement · 2025Article
- The Pentatricopeptide Repeat Protein OsPPR674 Regulates Rice Growth and Drought Sensitivity by Modulating RNA Editing of the Mitochondrial TranscriptInternational journal of molecular sciences · 2025Article
- Article
101 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cytoplasmic male sterility (CMS) phenotype in plants can be reversed by the action of nuclear-encoded fertility restorer (Rf) genes. The molecular mechanism involved in Rf gene-mediated processing of CMS-associated transcripts is unclear, as are the identities of other proteins that may be involved in the CMS-Rf interaction. In this study, we cloned the restorer gene Rf5 for Hong-Lian CMS in rice and studied its fertility restoration mechanism with respect to the processing of the CMS-associated transcript atp6-orfH79. RF5, a pentatricopeptide repeat (PPR) protein, was unable to bind to this CMS-associated transcript; however, a partner protein of RF5 (GRP162, a Gly-rich protein encoding 162 amino acids) was identified to bind to atp6-orfH79. GRP162 was found to physically interact with RF5 and to bind to atp6-orfH79 via an RNA recognition motif. Furthermore, we found that RF5 and GRP162 are both components of a restoration of fertility complex (RFC) that is 400 to 500 kD in size and can cleave CMS-associated transcripts in vitro. Evidence that a PPR protein interacts directly with a Gly-rich protein to form a subunit of the RFC provides a new perspective on the molecular mechanisms underlying fertility restoration.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.