ArticleApplied and environmental microbiology2012
Site-specific recombination strategies for engineering actinomycete genomes.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 100 citations in OpenAlex.
- Gene amir_2071 of Actinosynnema mirum DSM 43827 encodes a dimethylallyltryptophan synthase superfamily protein responsible for the production of prenylated tyrosine.Folia microbiologica · 2026Article
- Evaluation of vector system in Saccharopolyspora erythraea and construction of new replicative vector.Applied microbiology and biotechnology · 2026Article
- Genetically engineered Streptomyces viridosporus ATCC 14672 strains for the discovery of novel moenomycins.Scientific reports · 2026Article
- A Strictly Inducible and Orthogonal Dre-rox System for Precise and Markerless Genome Editing inJournal of microbiology and biotechnology · 2025Article
- Identification of a critical gene involved in the biosynthesis of the polyene macrolide lavencidin inApplied and environmental microbiology · 2025Article
- Mitigating genetic instability caused by the excision activity of theApplied and environmental microbiology · 2025Article
- Uncovering the genetic basis of antiviral polyketide limocrocin biosynthesis through heterologous expression.Microbial cell factories · 2025Article
- Current Approaches for Genetic Manipulation ofBiotech (Basel (Switzerland)) · 2025Review
- Bacterial genome engineering using CRISPR-associated transposases.Nature protocols · 2024Review
- The Impact of Heterologous Regulatory Genes from Lipodepsipeptide Biosynthetic Gene Clusters on the Production of Teicoplanin and A40926.Antibiotics (Basel, Switzerland) · 2024Article
- Genomic modifications for enhanced antibiotic production in rifamycin derivative-producingFrontiers in antibiotics · 2024Article
- Discovery and overproduction of novel highly bioactive pamamycins through transcriptional engineering of the biosynthetic gene cluster.Microbial cell factories · 2023Article
- Properties of Multidrug-Resistant Mutants Derived from Heterologous Expression Chassis StrainMicroorganisms · 2023Article
- Bacterial genome engineering using CRISPR RNA-guided transposases.bioRxiv : the preprint server for biology · 2023Article
- Landscape of Post-Transcriptional tRNA Modifications in Streptomyces albidoflavus J1074 as Portrayed by Mass Spectrometry and Genomic Data Mining.Journal of bacteriology · 2023Article
- Cloning and Expression of Metagenomic DNA in Streptomyces lividans and Its Subsequent Fermentation for Optimized Production.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Past, Present, and Future of Genome Modification inMicroorganisms · 2022Review
- Marker-Free Genome Engineering inMicroorganisms · 2022Article
- Genetic approaches to improve clorobiocin production in Streptomyces roseochromogenes NRRL 3504.Applied microbiology and biotechnology · 2022Article
- Genetic Engineering ofMicroorganisms · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The feasibility of using technologies based on site-specific recombination in actinomycetes was shown several years ago. Despite their huge potential, these technologies mostly have been used for simple marker removal from a chromosome. In this paper, we present different site-specific recombination strategies for genome engineering in several actinomycetes belonging to the genera Streptomyces, Micromonospora, and Saccharothrix. Two different systems based on Cre/loxP and Dre/rox have been utilized for numerous applications. The activity of the Cre recombinase on the heterospecific loxLE and loxRE sites was similar to its activity on wild-type loxP sites. Moreover, an apramycin resistance marker flanked by the loxLERE sites was eliminated from the Streptomyces coelicolor M145 genome at a surprisingly high frequency (80%) compared to other bacteria. A synthetic gene encoding the Dre recombinase was constructed and successfully expressed in actinomycetes. We developed a marker-free expression method based on the combination of phage integration systems and site-specific recombinases. The Cre recombinase has been used in the deletion of huge genomic regions, including the phenalinolactone, monensin, and lipomycin biosynthetic gene clusters from Streptomyces sp. strain Tü6071, Streptomyces cinnamonensis A519, and Streptomyces aureofaciens Tü117, respectively. Finally, we also demonstrated the site-specific integration of plasmid and cosmid DNA into the chromosome of actinomycetes catalyzed by the Cre recombinase. We anticipate that the strategies presented here will be used extensively to study the genetics of actinomycetes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.