ArticleApplied and environmental microbiology2012
A novel restriction-modification system is responsible for temperature-dependent phage resistance in Listeria monocytogenes ECII.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Targeting Foodborne Pathogens with Bacteriophages: Mechanisms, Applications, and Resistance.Pathogens (Basel, Switzerland) · 2026Review
- Multigenerational proteolytic inactivation of restriction upon subtle genomic hypomethylation in Pseudomonas aeruginosa.Nature microbiology · 2025Article
- The Virulence Factor LLO ofFoods (Basel, Switzerland) · 2025Article
- Article
- Identification and Characterization of a Novel Prophage Lysin againstMolecules (Basel, Switzerland) · 2024Article
- Biological Characterization and Genomic Analysis of Three NovelInternational journal of molecular sciences · 2024Article
- Anaerobiosis, a neglected factor in phage-bacteria interactions.Applied and environmental microbiology · 2023Article
- Characterization and Genome Study of a Newly Isolated Temperate Phage Belonging to a New Genus TargetingGenes · 2023Article
- Investigation of aMicroorganisms · 2023Article
- Manufacturing Bacteriophages (Part 1 of 2): Cell Line Development, Upstream, and Downstream Considerations.Pharmaceuticals (Basel, Switzerland) · 2021Review
- Whole Genome Sequence Analysis of Phage-ResistantPathogens (Basel, Switzerland) · 2021Article
- Inhibition of Antimicrobial-ResistantFrontiers in microbiology · 2021Article
- Effectiveness of Phage-Based Inhibition ofMicroorganisms · 2020Review
- Genomic dissection of the most prevalent Listeria monocytogenes clone, sequence type ST87, in China.BMC genomics · 2019Article
- Phage Therapy: What Have We Learned?Viruses · 2018Review
- Article
- The ProbioticFrontiers in microbiology · 2018Article
- Convergence of DNA methylation and phosphorothioation epigenetics in bacterial genomes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Novel Cadmium Resistance Determinant in Listeria monocytogenes.Applied and environmental microbiology · 2017Article
- Temperature Significantly Affects the Plaquing and Adsorption Efficiencies of Listeria Phages.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes epidemic clone II (ECII) strains are unusual in being completely resistant to phage when grown at low temperatures (≤30°C). In the current study we constructed and characterized a mariner-based mutant (J46C) of the ECII strain H7550-Cd(S) that lacked temperature-dependent resistance to phage. The transposon was localized in LMOh7858_2753 (open reading frame [ORF] 2753), a member of a 12-ORF genomic island unique to ECII strains. ORF 2753 and ORF 2754 exhibited homologies to restriction endonucleases and methyltransferases associated with type II restriction-modification (RM) systems. In silico-based predictions of the recognition site for this putative RM system were supported by resistance of DNA from ECII strains to digestion by BfuI, a type II restriction enzyme specific for GTATCC (N6/5). Similarly to J46C, a mutant harboring an in-frame deletion of ORF 2753 was susceptible to phage regardless of temperature of growth (25°C or 37°C). Genetic complementation restored phage resistance in 25°C-grown cells of ORF 2753 mutants. Reverse transcription (RT) and quantitative real-time PCR data suggested enhanced transcription of ORF 2753 at low temperatures (≤25°C) compared to 37°C. In contrast, available transcriptional data suggested that the putative methyltransferase (ORF 2754) was constitutively expressed at all tested temperatures (4 to 37°C). Thus, temperature-dependent resistance of L. monocytogenes ECII to phage is mediated by temperature-dependent expression of the restriction endonuclease associated with a novel RM system (LmoH7) unique to this epidemic clone.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.