ArticleApplied and environmental microbiology2012
Phenotypic switching in Pseudomonas brassicacearum involves GacS- and GacA-dependent Rsm small RNAs.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 58 citations in OpenAlex.
- TheApplied and environmental microbiology · 2026Article
- Modeling binding of the conserved Csr/Rsm protein family across species of the γ-proteobacteria reveals niche-specific adaptation of the post-transcriptional regulon.bioRxiv : the preprint server for biology · 2026Article
- DNA duplication in Burkholderia thailandensis induces biofilm formation by activating a two-component regulatory system.PLoS genetics · 2025Article
- Expression regulation of bacterial lipase genes: a review.Frontiers in microbiology · 2025Review
- DNA duplication-mediated activation of a two-component regulatory system serves as a bet-hedging strategy forbioRxiv : the preprint server for biology · 2024Article
- Quorum sensing-related activities of beneficial and pathogenic bacteria have important implications for plant and human health.FEMS microbiology ecology · 2024Review
- Dynamic nitrogen fixation in an aerobic endophyte of Populus.The ISME journal · 2024Article
- Phytobeneficial traits of rhizobacteria under the control of multiple molecular dialogues.Microbial biotechnology · 2022Article
- Review
- Importance ofPathogens (Basel, Switzerland) · 2021Article
- Delving into defence: identifying theMicrobial genomics · 2021Article
- Exploring the expression and functionality of theRNA biology · 2021Article
- Evolutionary history expands the range of signaling interactions in hybrid multikinase networks.Scientific reports · 2021Article
- Amplifying and Fine-Tuning Rsm sRNAs Expression and Stability to Optimize the Survival ofMicroorganisms · 2021Article
- The Evanescent GacS Signal.Microorganisms · 2020Review
- Genetic factors involved in rhizosphere colonization by phytobeneficialComputational and structural biotechnology journal · 2020Review
- Discovery of an Antibiotic-Related Small Protein of Biocontrol StrainFrontiers in microbiology · 2020Article
- Comparative Genomics and Evolutionary Analysis of RNA-Binding Proteins of the CsrA Family in the GenusFrontiers in molecular biosciences · 2020Article
- Polyamine is a critical determinant of Pseudomonas chlororaphis O6 for GacS-dependent bacterial cell growth and biocontrol capacity.Molecular plant pathology · 2018Article
- Enhanced Fluorescent Siderophore Biosynthesis and Loss of Phenazine-1-Carboxamide in Phenotypic Variant ofFrontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant-beneficial bacterium Pseudomonas brassicacearum forms phenotypic variants in vitro as well as in planta during root colonization under natural conditions. Transcriptome analysis of typical phenotypic variants using microarrays containing coding as well as noncoding DNA fragments showed differential expression of several genes relevant to secondary metabolism and of the small RNA (sRNA) genes rsmX, rsmY, and rsmZ. Naturally occurring mutations in the gacS-gacA system accounted for phenotypic switching, which was characterized by downregulation of antifungal secondary metabolites (2,4-diacetylphloroglucinol and cyanide), indoleacetate, exoenzymes (lipase and protease), and three different N-acyl-homoserine lactone molecules. Moreover, in addition to abrogating these biocontrol traits, gacS and gacA mutations resulted in reduced expression of the type VI secretion machinery, alginate biosynthesis, and biofilm formation. In a gacA mutant, the expression of rsmX was completely abolished, unlike that of rsmY and rsmZ. Overexpression of any of the three sRNAs in the gacA mutant overruled the pleiotropic changes and restored the wild-type phenotypes, suggesting functional redundancy of these sRNAs. In conclusion, our data show that phenotypic switching in P. brassicacearum results from mutations in the gacS-gacA system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.