ArticleNature2012
A novel sensor to map auxin response and distribution at high spatio-temporal resolution.
Article in Nature, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 328 papers.
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Who cites it
328 citing papers in PubMed.
- Quantitative Measurement of Basipetal Auxin Transport in Arabidopsis Roots ViaMethods in molecular biology (Clifton, N.J.) · 2027Article
- Unlocking the full potential of spatial omics in plants: practical challenges, solutions, and a path forward.The Plant cell · 2026Review
- Nano-flow liquid chromatography-mass spectrometry for highly sensitive quantification of indole-3-acetic acid from small tissue sections prepared by laser micro dissection.Journal of plant research · 2026Article
- Eukaryotic chaperonin coordinates root meristem activity by regulating SEC31B-dependent COPII vesicle trafficking of PIN auxin efflux carrier in Arabidopsis.Journal of integrative plant biology · 2026Article
- Robust quantification of multiplexed fluorescent protein-based biosensors in plant tissues.The Plant journal : for cell and molecular biology · 2026Article
- Development of the HTRF assay to evaluate the auxin-induced binding between TIR1 and IAA7.The New phytologist · 2026Article
- Thermosensory reconfiguration of the auxin transcriptional pathway to drive root cell growth.Nature communications · 2026Article
- In Vivo Detection of IAA Using IAA Nanosensor.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Phytohormonal regulation of root exudation: mechanisms and rhizosphere function.Plant signaling & behavior · 2025Review
- Article
- An RbohC and RbohE-mediated ROS oscillatory circuit regulates root cap turnover in Arabidopsis.iScience · 2025Article
- Florigen and cytokinin signaling antagonistically regulate FLOWERING LOCUS T-LIKE1 to drive a florigen relay that facilitates inflorescence development in rice.Science advances · 2025Article
- Carbon source utilization regulates biofilm formation and plant-beneficial interactions ofiScience · 2025Article
- Angle dependence as a unifying feature of root graviresponse modules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- HSP90 differentially stabilizes plant ABCB-type auxin transporters on the plasma membrane.Nature communications · 2025Article
- Annual Dynamics of Endogenous Hormones Reveal the Mechanism of Off-Season Flowering in Macadamia.Plants (Basel, Switzerland) · 2025Article
- Cytosolic- and membrane-localized oxidized indole-3-acetic acid formation regulates developmental auxin transients.Plant physiology · 2025Article
- Enhanced auxin signaling promotes root-hair growth at moderately low temperature in Arabidopsis thaliana.Plant communications · 2025Article
- Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits.The Plant cell · 2025Article
- CUC/auxin patterning of decanalised petal number inQuantitative plant biology · 2025Article
268 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Auxin is a key plant morphogenetic signal but tools to analyse dynamically its distribution and signalling during development are still limited. Auxin perception directly triggers the degradation of Aux/IAA repressor proteins. Here we describe a novel Aux/IAA-based auxin signalling sensor termed DII-VENUS that was engineered in the model plant Arabidopsis thaliana. The VENUS fast maturing form of yellow fluorescent protein was fused in-frame to the Aux/IAA auxin-interaction domain (termed domain II; DII) and expressed under a constitutive promoter. We initially show that DII-VENUS abundance is dependent on auxin, its TIR1/AFBs co-receptors and proteasome activities. Next, we demonstrate that DII-VENUS provides a map of relative auxin distribution at cellular resolution in different tissues. DII-VENUS is also rapidly degraded in response to auxin and we used it to visualize dynamic changes in cellular auxin distribution successfully during two developmental responses, the root gravitropic response and lateral organ production at the shoot apex. Our results illustrate the value of developing response input sensors such as DII-VENUS to provide high-resolution spatio-temporal information about hormone distribution and response during plant growth and development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.