ArticleDevelopment genes and evolution2012
Optimized conditions for transgenesis of the ascidian Ciona using square wave electroporation.
Article in Development genes and evolution, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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5 citing papers in PubMed, 17 citations in OpenAlex.
- Quantitative Dissection of the ProximalFrontiers in cell and developmental biology · 2021Article
- The impact of non-electrical factors on electrical gene transfer.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Parallel evolution of chordate cis-regulatory code for development.PLoS genetics · 2013Article
- Study of Cis-regulatory Elements in the Ascidian Ciona intestinalis.Current genomics · 2013Article
- Purification of mitochondrial proteins HSP60 and ATP synthase from ascidian eggs: implications for antibody specificity.PloS one · 2013Article
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2 authors at 1 institution in 1 country.
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Abstract
The protochordate Ciona has recently become an important model organism for the study of developmental gene regulation, in part because transient transgenic embryos can be produced rapidly and reliably using electroporation. Published methods are currently for the use of electroporation devices delivering exponentially decaying pulses. However, some workers have advocated the use of square wave electroporation devices for eukaryotic transgenics. This paper presents results of experiments to find optimal conditions for the use of square-wave electroporation in the ascidian Ciona. In the present analysis, a single pulse of 90 msec duration at 63-75 V/cm was found to give an optimal balance of a high proportion of cells transformed with the transgene, and a low level of abnormal development. Forty micrograms of DNA per electroporation is sufficient for effective visualization of reporter gene expression, although doses up to 100 μg provide higher proportions of transformed cells. In side-by-side comparison with exponential pulse electroporation, square wave pulses give similar penetrance of transgene expression, along with lower proportions of embryos with abnormal development at higher amounts of input DNA.
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