ArticleJournal of structural biology2012
Chemical mapping of mammalian cells by atom probe tomography.
Article in Journal of structural biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 44 citations in OpenAlex.
- Nanoscale analysis of frozen honey by atom probe tomography.Scientific reports · 2022Article
- The homogenous alternative to biomineralization: Zn- and Mn-rich materials enable sharp organismal "tools" that reduce force requirements.Scientific reports · 2021Article
- Atom probe tomography.Nature reviews. Methods primers · 2021Article
- Enabling near-atomic-scale analysis of frozen water.Science advances · 2020Article
- Field evaporation and atom probe tomography of pure water tips.Scientific reports · 2020Article
- New frontiers in atom probe tomography: a review of research enabled by cryo and/or vacuum transfer systems.Materials today. Advances · 2020Article
- Atomic layout of an orthodontic titanium mini-implant in human tissue: Insights into the possible mechanisms during osseointegration.The Angle orthodontist · 2019Article
- A near atomic-scale view at the composition of amyloid-beta fibrils by atom probe tomography.Scientific reports · 2018Article
- The advent of structural biologyBiophysics reports · 2017Article
- Near-Atomic Three-Dimensional Mapping for Site-Specific Chemistry of 'Superbugs'.Nano letters · 2016Article
- Focused ion beams in biology.Nature methods · 2015Review
- Progress toward single cell metabolomics.Current opinion in biotechnology · 2013Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
In atom probe tomography (APT), a technique that has been used to determine 3D maps of ion compositions of metals and semiconductors at sub-nanometer resolutions, controlled emissions of ions can be induced from needle-shaped specimens in the vicinity of a strong electric field. Detection of these ions in the plane of a position sensitive detector provides two-dimensional compositional information while the sequence of ion arrival at the detector provides information in the third dimension. Here we explore the use of APT technology for imaging biological specimens. We demonstrate that it is possible to obtain 3D spatial distributions of cellular ions and metabolites from unstained, freeze-dried mammalian cells. Multiple peaks were reliably obtained in the mass spectrum from tips with diameters of ~50 nm and heights of ~200 nm, with mass-to-charge ratios (m/z) ranging from 1 to 80. Peaks at m/z 12, 23, 28 and 39, corresponding to carbon, sodium, carbonyl and potassium ions respectively, showed distinct patterns of spatial distribution within the cell. Our studies establish that APT could become a powerful tool for mapping the sub-cellular distribution of atomic species, such as labeled metabolites, at 3D spatial resolutions as high as ~1 nm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.