ArticleBiomaterials2012
The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds.Journal of visualized experiments : JoVE · 2025Article
- Article
- Hyper-Branched Cationic Cyclodextrin Polymers for Improving Plasmid Transfection in 2D and 3D Spheroid Cells.Pharmaceutics · 2022Article
- Article
- Growth environment influences B16.F10 mouse melanoma cell response to gene electrotransfer.Bioelectrochemistry (Amsterdam, Netherlands) · 2021Article
- 3D Bioprinted cancer models: Revolutionizing personalized cancer therapy.Translational oncology · 2021Review
- The Efficiency of Gene Electrotransfer in Breast-Cancer Cell Lines Cultured on a Novel Collagen-Free 3D Scaffold.Cancers · 2020Article
- Calcium Delivery by Electroporation Induces In Vitro Cell Death through Mitochondrial Dysfunction without DNA Damages.Cancers · 2020Article
- 3D-printing enabled micro-assembly of a microfluidic electroporation system for 3D tissue engineering.Lab on a chip · 2019Article
- Upregulation of DNA Sensors in B16.F10 Melanoma Spheroid Cells After Electrotransfer of pDNA.Technology in cancer research & treatment · 2018Article
- Recellularized human dermis for testing gene electrotransfer ex vivo.Biomedical materials (Bristol, England) · 2016Article
- Article
- 3D spheroids' sensitivity to electric field pulses depends on their size.The Journal of membrane biology · 2013Article
- Transfection in the third dimension.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- In vitro-in vivo translation of lipid nanoparticles for hepatocellular siRNA delivery.ACS nano · 2012Article
- Bioreactor-Based Tumor Tissue Engineering.Acta naturaeArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
A large-scale in vitro 3D tumor model was generated to evaluate gene delivery procedures in vivo. This 3D tumor model consists of a "tissue-like" spheroid that provides a micro-environment supportive of melanoma proliferation, allowing cells to behave similarly to cells in vivo. This functional spheroid measures approximately 1 cm in diameter and can be used to effectively evaluate plasmid transfection when testing various electroporation (EP) electrode applicators. In this study, we identified EP conditions that efficiently transfect green fluorescent protein (GFP) and interleukin 15 (IL-15) plasmids into tumor cells residing in the 3D construct. We found that plasmids delivered using a 6-plate electrode applying 6 pulses with nominal electric field strength of 500 V/cm and pulse-length of 20 ms produced significant increase of GFP (7.3-fold) and IL-15 (3.0-fold) expression compared to controls. This in vitro 3D model demonstrates the predictability of cellular response toward delivery techniques, limits the numbers of animals employed for transfection studies, and may facilitate future developments of clinical trials for cancer therapies in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.