ArticleMicrobiology (Reading, England)2012
NhaP1 is a K+(Na+)/H+ antiporter required for growth and internal pH homeostasis of Vibrio cholerae at low extracellular pH.
Article in Microbiology (Reading, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- The evolution of a NaJournal of bacteriology · 2026Article
- The evolution of a NabioRxiv : the preprint server for biology · 2026Article
- HadS, a membrane-associated 2-haloacid dehalogenase from the moderate halophileFrontiers in microbiology · 2026Article
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- Characterization of Two NaInternational journal of molecular sciences · 2023Article
- Vibrio cholerae RND efflux systems: mediators of stress responses, colonization and pathogenesis.Frontiers in cellular and infection microbiology · 2023Review
- Quorum Sensing Controls the CRISPR and Type VI Secretion Systems inFrontiers in veterinary science · 2022Article
- Vibrio cholerae OmpR Contributes to Virulence Repression and Fitness at Alkaline pH.Infection and immunity · 2020Article
- Crosstalks Between Gut Microbiota andFrontiers in cellular and infection microbiology · 2020Review
- Physiological, Structural, and Functional Analysis of the Paralogous Cation-Proton Antiporters of NhaP Type fromInternational journal of molecular sciences · 2019Review
- Implications for Cation Selectivity and Evolution by a Novel Cation Diffusion Facilitator Family Member From the Moderate HalophileFrontiers in microbiology · 2019Article
- An Uncharacterized Major Facilitator Superfamily Transporter FromFrontiers in microbiology · 2018Article
- Characterization of a Functionally Unknown Arginine-Aspartate-Aspartate Family Protein FromFrontiers in microbiology · 2018Article
- Characterization of a novel two-component NaScientific reports · 2017Article
- A UPF0118 family protein with uncharacterized function from the moderate halophile Halobacillus andaensis represents a novel class of NaScientific reports · 2017Article
- Physiology of the Vc-NhaP paralogous group of cation-proton antiporters in Vibrio cholerae.Molecular and cellular biochemistry · 2017Article
- Effects of chromosomal deletion of the operon encoding the multiple resistance and pH-related antiporter in Vibrio cholerae.Microbiology (Reading, England) · 2016Article
- Cloning and identification of Group 1 mrp operon encoding a novel monovalent cation/proton antiporter system from the moderate halophile Halomonas zhaodongensis.Extremophiles : life under extreme conditions · 2014Article
- The C-terminal cytoplasmic portion of the NhaP2 cation-proton antiporter from Vibrio cholerae affects its activity and substrate affinity.Molecular and cellular biochemistry · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Vibrio cholerae has adapted to a wide range of salinity, pH and osmotic conditions, enabling it to survive passage through the host and persist in the environment. Among the many proteins responsible for bacterial survival under these diverse conditions, we have identified Vc-NhaP1 as a K(+)(Na(+))/H(+) antiporter essential for V. cholerae growth at low environmental pH. Deletion of the V. cholerae nhaP1 gene caused growth inhibition when external potassium was either limited (100 mM and below) or in excess (400 mM and above). This growth defect was most apparent at mid-exponential phase, after 4-6 h of culture. Using a pH-sensitive GFP, cytosolic pH was shown to be dependent on K(+) in acidic external conditions in a Vc-NhaP1-dependent manner. When functionally expressed in an antiporterless Escherichia coli strain and assayed in everted membrane vesicles, Vc-NhaP1 operated as an electroneutral alkali cation/proton antiporter, exchanging K(+) or Na(+) ions for H(+) within a broad pH range (7.25-9.0). These data establish the putative V. cholerae NhaP1 protein as a functional K(+)(Na(+))/H(+) antiporter of the CPA1 family that is required for bacterial pH homeostasis and growth in an acidic environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.