ArticleNature protocols2012
Intravital multiphoton imaging of immune responses in the mouse ear skin.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 91 papers.
What it found
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Who cites it
91 citing papers in PubMed.
- Individual cell motion in healthy human skin microvasculature by reflectance confocal video microscopy.Microcirculation (New York, N.Y. : 1994) · 2020Trial
- Cross-species comparative spatial transcriptomics of hair follicle-T cell interactions identifies conserved drivers of cutaneous lupus erythematosus skin disease and associated hair loss.Nature communications · 2026Article
- Spatially Distinct Myosin II Architectures Regulate Protrusion Dynamics and Directional Persistence during Immune Cell Migration.bioRxiv : the preprint server for biology · 2026Article
- Intravital imaging of the formation and resolution of MHC class II-positive T-cell activation niches.Life science alliance · 2026Article
- Long-wavelength oblique back-illumination microscopy for deepBiomedical optics express · 2026Article
- Enhanced nanoparticle delivery across vascular basement membranes of tumours using nitric oxide.Nature biomedical engineering · 2025Article
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- Fluorescent Particles Based on Aggregation-Induced Emission for Optical Diagnostics of the Central Nervous System.Research (Washington, D.C.) · 2025Review
- General Post-Regulation Strategy of AIEgens' Photophysical Properties for Intravital Two-Photon Fluorescence Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Noninvasive in vivo microscopy of single neutrophils in the mouse brain via NIR-II fluorescent nanomaterials.Nature protocols · 2024Review
- Compact simultaneous label-free autofluorescence multi-harmonic microscopy for user-friendly photodamage-monitored imaging.Journal of biomedical optics · 2024Article
- Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Melittin-Carrying Nanoparticle Suppress T Cell-Driven Immunity in a Murine Allergic Dermatitis Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Apoptotic extracellular vesicles are metabolized regulators nurturing the skin and hair.Bioactive materials · 2023Article
- Article
- Spatiotemporal dynamics of calcium signals during neutrophil cluster formation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Real-time observation of neutrophil extracellular trap formation in the inflamed mouse brain via two-photon intravital imaging.Laboratory animal research · 2022Review
- Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions.Journal of visualized experiments : JoVE · 2022Article
- Non-invasive longitudinal imaging of VEGF-induced microvascular alterations in skin wounds.Theranostics · 2022Article
- Epigenetic basis of diabetic vasculopathy.Frontiers in endocrinology · 2022Review
31 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiphoton (MP) microscopy enables the direct in vivo visualization, with high spatial and temporal resolution, of fluorescently tagged immune cells, extracellular matrix and vasculature in tissues. This approach, therefore, represents a powerful alternative to traditional methods of assessing immune cell function in the skin, which are mainly based on flow cytometry and histology. Here we provide a step-by-step protocol describing experimental procedures for intravital MP imaging of the mouse ear skin, which can be easily adapted to address many specific skin-related biological questions. We demonstrate the use of this procedure by characterizing the response of neutrophils during cutaneous inflammation, which can be used to perform in-depth analysis of neutrophil behavior in the context of the skin microanatomy, including the epidermis, dermis and blood vessels. Such experiments are typically completed within 1 d, but as the procedures are minimally invasive, it is possible to perform longitudinal studies through repeated imaging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.