ArticleBiomaterials2012
Phosphorescent nanoparticles for quantitative measurements of oxygen profiles in vitro and in vivo.
Article in Biomaterials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications.Chemical reviews · 2024Review
- Rigor and reproducibility in human brain organoid research: Where we are and where we need to go.Stem cell reports · 2024Review
- Article
- Heterosubstituted Derivatives of PtPFPP for OBioconjugate chemistry · 2022Article
- Ru(ii)/BODIPY core co-encapsulated ratiometric nanotools for intracellular ORSC chemical biology · 2021Article
- Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids.Nature communications · 2021Article
- Review: Nanomaterials for Reactive Oxygen Species Detection and Monitoring in Biological Environments.Frontiers in chemistry · 2021Review
- Biological reduction of nitroimidazole-functionalized gold nanorods for photoacoustic imaging of tumor hypoxia.RSC advances · 2019Article
- Oxyphor 2P: A High-Performance Probe for Deep-Tissue Longitudinal Oxygen Imaging.Cell metabolism · 2019Article
- Two-photon dual imaging platform for in vivo monitoring cellular oxidative stress in liver injury.Scientific reports · 2017Article
- Two-Photon Absorbing Phosphorescent Metalloporphyrins: Effects of π-Extension and Peripheral Substitution.Journal of the American Chemical Society · 2016Article
- Oxygen Tension and Riboflavin Gradients Cooperatively Regulate the Migration ofFrontiers in microbiology · 2016Article
- Ratiometric Molecular Probes Based on Dual Emission of a Blue Fluorescent Coumarin and a Red Phosphorescent Cationic Iridium(III) Complex for Intracellular Oxygen Sensing.Sensors (Basel, Switzerland) · 2015Article
- Collagen-based brain microvasculature model in vitro using three-dimensional printed template.Biomicrofluidics · 2015Article
- Combined two-photon excitation and d→f energy transfer in a water-soluble Ir(III)/Eu(III) dyad: two luminescence components from one molecule for cellular imaging.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- Two-photon antenna-core oxygen probe with enhanced performance.Analytical chemistry · 2014Article
- Dinuclear ruthenium(II) complexes as two-photon, time-resolved emission microscopy probes for cellular DNA.Angewandte Chemie (International ed. in English) · 2014Article
- Cell microenvironment engineering and monitoring for tissue engineering and regenerative medicine: the recent advances.BioMed research international · 2014Review
- Indicators for optical oxygen sensors.Bioanalytical reviews · 2012Article
- Microfluidics for mammalian cell chemotaxis.Annals of biomedical engineering · 2012Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
We present the development and characterization of nanoparticles loaded with a custom phosphor; we exploit these nanoparticles to perform quantitative measurements of the concentration of oxygen within three-dimensional (3-D) tissue cultures in vitro and blood vessels in vivo. We synthesized a customized ruthenium (Ru)-phosphor and incorporated it into polymeric nanoparticles via self-assembly. We demonstrate that the encapsulated phosphor is non-toxic with and without illumination. We evaluated two distinct modes of employing the phosphorescent nanoparticles for the measurement of concentrations of oxygen: 1) in vitro, in a 3-D microfluidic tumor model via ratiometric measurements of intensity with an oxygen-insensitive fluorophore as a reference, and 2) in vivo, in mouse vasculature using measurements of phosphorescence lifetime. With both methods, we demonstrated micrometer-scale resolution and absolute calibration to the dissolved oxygen concentration. Based on the ease and customizability of the synthesis of the nanoparticles and the flexibility of their application, these oxygen-sensing polymeric nanoparticles will find a natural home in a range of biological applications, benefiting studies of physiological as well as pathological processes in which oxygen availability and concentration play a critical role.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.