ArticleThe Biochemical journal2012
Novel structural arrangement of nematode cystathionine β-synthases: characterization of Caenorhabditis elegans CBS-1.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- The transsulfuration pathway suppresses the embryonic lethal phenotype of glutathione reductase mutants in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2025Article
- TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation.The EMBO journal · 2024Article
- The coralScience advances · 2022Article
- Structural insight into the unique conformation of cystathionine β-synthase fromComputational and structural biotechnology journal · 2021Article
- Functional characterization of Caenorhabditis elegans cbs-2 gene during meiosis.Scientific reports · 2020Article
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- Lifespan and healthspan benefits of exogenous HNPJ aging and mechanisms of disease · 2020Article
- Prostaglandin signals from adult germ stem cells delay somatic aging ofNature metabolism · 2019Article
- A timeline of hydrogen sulfide (HBiochemical pharmacology · 2018Review
- Hydrogen sulfide is an endogenous regulator of aging in Caenorhabditis elegans.Antioxidants & redox signaling · 2014Article
- Regulation of mitochondrial bioenergetic function by hydrogen sulfide. Part II. Pathophysiological and therapeutic aspects.British journal of pharmacology · 2014Review
- Regulation of mitochondrial bioenergetic function by hydrogen sulfide. Part I. Biochemical and physiological mechanisms.British journal of pharmacology · 2014Review
- Purification, crystallization and preliminary crystallographic analysis of the catalytic core of cystathionine β-synthase from Saccharomyces cerevisiae.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Domain organization, catalysis and regulation of eukaryotic cystathionine beta-synthases.PloS one · 2014Article
- C. elegans aging is modulated by hydrogen sulfide and the sulfhydrylase/cysteine synthase cysl-2.PloS one · 2013Article
- Purification, crystallization and preliminary crystallographic analysis of human cystathionine β-synthase.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Purification, crystallization and preliminary crystallographic analysis of the full-length cystathionine β-synthase from Apis mellifera.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- The neurobiology of sensing respiratory gases for the control of animal behavior.Frontiers in biology · 2012Article
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5 authors at 1 institution in 1 country.
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Abstract
CBSs (cystathionine β-synthases) are eukaryotic PLP (pyridoxal 5 *-phosphate)-dependent proteins that maintain cellular homocysteine homoeostasis and produce cystathionine and hydrogen sulfide. In the present study, we describe a novel structural arrangement of the CBS enzyme encoded by the cbs-1 gene of the nematode Caenorhabditis elegans. The CBS-1 protein contains a unique tandem repeat of two evolutionarily conserved catalytic regions in a single polypeptide chain. These repeats include a catalytically active C-terminal module containing a PLP-binding site and a less conserved N-terminal module that is unable to bind the PLP cofactor and cannot catalyse CBS reactions, as demonstrated by analysis of truncated variants and active-site mutant proteins. In contrast with other metazoan enzymes, CBS-1 lacks the haem and regulatory Bateman domain essential for activation by AdoMet (S-adenosylmethionine) and only forms monomers. We determined the tissue and subcellular distribution of CBS-1 and showed that cbs-1 knockdown by RNA interference leads to delayed development and to an approximately 10-fold elevation of homocysteine concentrations in nematode extracts. The present study provides the first insight into the metabolism of sulfur amino acids and hydrogen sulfide in C. elegans and shows that nematode CBSs possess a structural feature that is unique among CBS proteins.
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