ArticleJournal of the American Chemical Society2012
Engineering biosensors with extended, narrowed, or arbitrarily edited dynamic range.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- tKeima: A Large-Stokes-Shift Platform for Metal Ion Detection.Biosensors · 2026Article
- Advancements and Prospects in DNA-Based Bioanalytical Technology for Environmental Toxicant Detection.JACS Au · 2025Review
- Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output.Nature nanotechnology · 2025Article
- Liquid NanoBiosensors Enable One-Pot Electrochemical Detection of Bacteria in Complex Matrices.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- A high-dimensional microfluidic approach for selection of aptamers with programmable binding affinities.Nature chemistry · 2023Article
- Functional advantages of building nanosystems using multiple molecular components.Nature chemistry · 2023Article
- The quantum dotChemical science · 2022Article
- Programming ultrasensitive threshold response through chemomechanical instability.Nature communications · 2021Article
- Unraveling the effect of the aptamer complementary element on the performance of duplexed aptamers: a thermodynamic study.Analytical and bioanalytical chemistry · 2021Article
- Electrochemical DNA Biosensor That Detects Early Celiac Disease Autoantibodies.Sensors (Basel, Switzerland) · 2021Article
- Expanding detection windows for discriminating single nucleotide variants using rationally designed DNA equalizer probes.Nature communications · 2020Article
- Re-Evaluating the Conventional Wisdom about Binding Assays.Trends in biochemical sciences · 2020Review
- Article
- Computational Design of an Allosteric Antibody Switch by Deletion and Rescue of a Complex Structural Constellation.ACS central science · 2020Article
- Single-step multivalent capture assay for nucleic acid detection with dual-affinity regulation using mutation inhibition and allosteric activation.Chemical science · 2019Article
- Article
- Tethered imidazole mediated duplex stabilization and its potential for aptamer stabilization.Nucleic acids research · 2018Article
- Label-Free Detection of Tear Biomarkers Using Hydrogel-Coated Gold Nanoshells in a Localized Surface Plasmon Resonance-Based Biosensor.ACS nano · 2018Article
- Aptamer Recognition of Multiplexed Small-Molecule-Functionalized Substrates.ACS applied materials & interfaces · 2018Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Biomolecular recognition has long been an important theme in artificial sensing technologies. A current limitation of protein- and nucleic acid-based recognition, however, is that the useful dynamic range of single-site binding typically spans an 81-fold change in target concentration, an effect that limits the utility of biosensors in applications calling for either great sensitivity (a steeper relationship between target concentration and output signal) or the quantification of more wide-ranging concentrations. In response, we have adapted strategies employed by nature to modulate the input-output response of its biorecognition systems to rationally edit the useful dynamic range of an artificial biosensor. By engineering a structure-switching mechanism to tune the affinity of a receptor molecule, we first generated a set of receptor variants displaying similar specificities but different target affinities. Using combinations of these receptor variants (signaling and nonsignaling), we then rationally extended (to 900000-fold), narrowed (to 5-fold), and edited (three-state) the normally 81-fold dynamic range of a representative biosensor. We believe that these strategies may be widely applicable to technologies reliant on biorecognition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.