ArticlePloS one2012
Repression of meiotic genes by antisense transcription and by Fkh2 transcription factor in Schizosaccharomyces pombe.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Article
- Long Non-Coding RNAs in the Control of Gametogenesis: Lessons from Fission Yeast.Non-coding RNA · 2021Review
- CDK Regulation of Meiosis: Lessons fromGenes · 2020Review
- Long noncoding RNA repertoire and targeting by nuclear exosome, cytoplasmic exonuclease, and RNAi in fission yeast.RNA (New York, N.Y.) · 2018Article
- Article
- Histone deacetylation promotes transcriptional silencing at facultative heterochromatin.Nucleic acids research · 2018Article
- Developmentally regulated internal transcription initiation during meiosis in budding yeast.PloS one · 2017Article
- The Role of Transcription Factors at Antisense-Expressing Gene Pairs in Yeast.Genome biology and evolution · 2016Article
- Cuf2 Is a Transcriptional Co-Regulator that Interacts with Mei4 for Timely Expression of Middle-Phase Meiotic Genes.PloS one · 2016Article
- An antisense RNA-mediated mechanism eliminates a meiosis-specific copper-regulated transcript in mitotic cells.The Journal of biological chemistry · 2015Article
- Natural genetic variation impacts expression levels of coding, non-coding, and antisense transcripts in fission yeast.Molecular systems biology · 2014Article
- Evolutionarily diverse determinants of meiotic DNA break and recombination landscapes across the genome.Genome research · 2014Article
- A novel histone deacetylase complex in the control of transcription and genome stability.Molecular and cellular biology · 2014Article
- A global non-coding RNA system modulates fission yeast protein levels in response to stress.Nature communications · 2014Article
- Complexities of gammaherpesvirus transcription revealed by microarrays and RNAseq.Current opinion in virology · 2013Review
- Trancriptional landscape of Aspergillus niger at breaking of conidial dormancy revealed by RNA-sequencing.BMC genomics · 2013Article
- A dominant role for meiosis-specific 3' RNA processing in controlling expression of a fission yeast cyclin gene.RNA (New York, N.Y.) · 2012Article
- Mmi1 RNA surveillance machinery directs RNAi complex RITS to specific meiotic genes in fission yeast.The EMBO journal · 2012Article
- Rhn1, a nuclear protein, is required for suppression of meiotic mRNAs in mitotically dividing fission yeast.PloS one · 2012Article
- A conserved regulatory role for antisense RNA in meiotic gene expression in yeast.Current opinion in microbiology · 2011Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
In S. pombe, about 5% of genes are meiosis-specific and accumulate little or no mRNA during vegetative growth. Here we use Affymetrix tiling arrays to characterize transcripts in vegetative and meiotic cells. In vegetative cells, many meiotic genes, especially those induced in mid-meiosis, have abundant antisense transcripts. Disruption of the antisense transcription of three of these mid-meiotic genes allowed vegetative sense transcription. These results suggest that antisense transcription represses sense transcription of meiotic genes in vegetative cells. Although the mechanism(s) of antisense mediated transcription repression need to be further explored, our data indicates that RNAi machinery is not required for repression. Previously, we and others used non-strand specific methods to study splicing regulation of meiotic genes and concluded that 28 mid-meiotic genes are spliced only in meiosis. We now demonstrate that the "unspliced" signal in vegetative cells comes from the antisense RNA, not from unspliced sense RNA, and we argue against the idea that splicing regulates these mid-meiotic genes. Most of these mid-meiotic genes are induced in mid-meiosis by the forkhead transcription factor Mei4. Interestingly, deletion of a different forkhead transcription factor, Fkh2, allows low levels of sense expression of some mid-meiotic genes in vegetative cells. We propose that vegetative expression of mid-meiotic genes is repressed at least two independent ways: antisense transcription and Fkh2 repression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.