ArticlePlant physiology2012
Laticifer-specific cis-prenyltransferase silencing affects the rubber, triterpene, and inulin content of Taraxacum brevicorniculatum.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 128 citations in OpenAlex.
- Laticifer-specific engineering of rubber biosynthesis inBiodesign research · 2026Article
- A Dual-Reporter System for the Analysis of Phloem Structural and Signaling Responses In Vivo.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Effect of salicylic acid on morphological traits and the expression of key rubber biosynthesis genes in Parthenium argentatum A. Gray.PloS one · 2026Article
- Simultaneous Knockout of Tk1-SST and Tk1-FFT via CRISPR/Cas9 Enhances the Natural Rubber Accumulation in Taraxacum kok-saghyz.Plant biotechnology journal · 2025Article
- TkSRPP3/4 interactors TkGGR1 and TkLIL3 link plastid-like organelles with isoprenoid metabolism in Taraxacum koksaghyz latex.Plant cell reports · 2025Article
- Rubber biosynthesis drives the biogenesis and development of rubber particles, the rubber-producing organelles.Plant biotechnology journal · 2025Article
- Characterization of cis-polyisoprene produced in Periploca sepium, a novel promising alternative source of natural rubber.Communications biology · 2025Article
- The cis-prenyltransferase protein family in Taraxacum koksaghyz.The Plant journal : for cell and molecular biology · 2025Article
- Physiological and gene expression analysis of Hevea brasiliensis under drought stress.PloS one · 2025Article
- Methyl jasmonate improves rubber production and quality in Lactuca Serriola.Scientific reports · 2024Article
- Review
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
- Effect of drought stress on natural rubber biosynthesis and quality in Taraxacum kok-saghyz roots.PloS one · 2024Article
- Advances in Genome Sequencing and Natural Rubber Biosynthesis in Rubber-Producing Plants.Current issues in molecular biology · 2023Review
- Molecular Genetic Research and Genetic Engineering ofPlants (Basel, Switzerland) · 2023Review
- Identification of miRNAs and their targets in twoFrontiers in plant science · 2023Article
- Overexpression of a pseudo-etiolated-in-light-like protein inFrontiers in plant science · 2023Article
- Trehalose 6-Phosphate/SnRK1 Signaling Participates in Harvesting-Stimulated Rubber Production in the Hevea Tree.Plants (Basel, Switzerland) · 2022Article
- Reconstitution of prenyltransferase activity on nanodiscs by components of the rubber synthesis machinery of the Para rubber tree and guayule.Scientific reports · 2022Article
- Genetic transformation technologies for the common dandelion, Taraxacum officinale.Plant methods · 2021Article
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Certain Taraxacum species, such as Taraxacum koksaghyz and Taraxacum brevicorniculatum, produce large amounts of high-quality natural rubber in their latex, the milky cytoplasm of specialized cells known as laticifers. This high-molecular mass biopolymer consists mainly of poly(cis-1,4-isoprene) and is deposited in rubber particles by particle-bound enzymes that carry out the stereospecific condensation of isopentenyl diphosphate units. The polymer configuration suggests that the chain-elongating enzyme (rubber transferase; EC 2.5.1.20) is a cis-prenyltransferase (CPT). Here, we present a comprehensive analysis of transgenic T. brevicorniculatum plants in which the expression of three recently isolated CPTs known to be associated with rubber particles (TbCPT1 to -3) was heavily depleted by laticifer-specific RNA interference (RNAi). Analysis of the CPT-RNAi plants by nuclear magnetic resonance, size-exclusion chromatography, and gas chromatography-mass spectrometry indicated a significant reduction in rubber biosynthesis and a corresponding 50% increase in the levels of triterpenes and the main storage carbohydrate, inulin. Transmission electron microscopy revealed that the laticifers in CPT-RNAi plants contained fewer and smaller rubber particles than wild-type laticifers. We also observed lower activity of hydroxymethylglutaryl-coenzyme A reductase, the key enzyme in the mevalonate pathway, reflecting homeostatic control of the isopentenyl diphosphate pool. To our knowledge, this is the first in planta demonstration of latex-specific CPT activity in rubber biosynthesis.
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