ArticleNucleic acids research2012
A novel phage-encoded transcription antiterminator acts by suppressing bacterial RNA polymerase pausing.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Replication cycle timing determines phage sensitivity to a cytidine deaminase toxin/antitoxin bacterial defense system.PLoS pathogens · 2023Article
- Time to lysis determines phage sensitivity to a cytidine deaminase toxin/antitoxin bacterial defense system.bioRxiv : the preprint server for biology · 2023Article
- Development of ONT-cappable-seq to unravel the transcriptional landscape ofComputational and structural biotechnology journal · 2022Article
- Control of a programmed cell death pathway in Pseudomonas aeruginosa by an antiterminator.Nature communications · 2021Article
- The σ Subunit-Remodeling Factors: An Emerging Paradigms of Transcription Regulation.Frontiers in microbiology · 2020Review
- The Mechanisms of Substrate Selection, Catalysis, and Translocation by the Elongating RNA Polymerase.Journal of molecular biology · 2019Review
- Structural basis for transcription antitermination at bacterial intrinsic terminator.Nature communications · 2019Article
- A Thermus phage protein inhibits host RNA polymerase by preventing template DNA strand loading during open promoter complex formation.Nucleic acids research · 2018Article
- Mutations in the CRE pocket of bacterial RNA polymerase affect multiple steps of transcription.Nucleic acids research · 2015Article
- N protein from lambdoid phages transforms NusA into an antiterminator by modulating NusA-RNA polymerase flap domain interactions.Nucleic acids research · 2015Article
- Distinct pathways of RNA polymerase regulation by a phage-encoded factor.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- A bacteriophage transcription regulator inhibits bacterial transcription initiation by σ-factor displacement.Nucleic acids research · 2014Article
- Structural basis for promoter specificity switching of RNA polymerase by a phage factor.Genes & development · 2014Article
- Molecular basis of RNA polymerase promoter specificity switch revealed through studies ofBacteriophage · 2014Article
- The interaction surface of a bacterial transcription elongation factor required for complex formation with an antiterminator during transcription antitermination.The Journal of biological chemistry · 2013Article
- Transcription regulation mechanisms of bacteriophages: recent advances and future prospects.BioengineeredReview
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Gp39, a small protein encoded by Thermus thermophilus phage P23-45, specifically binds the host RNA polymerase (RNAP) and inhibits transcription initiation. Here, we demonstrate that gp39 also acts as an antiterminator during transcription through intrinsic terminators. The antitermination activity of gp39 relies on its ability to suppress transcription pausing at poly(U) tracks. Gp39 also accelerates transcription elongation by decreasing RNAP pausing and backtracking but does not significantly affect the rates of catalysis of individual reactions in the RNAP active center. We mapped the RNAP-gp39 interaction site to the β flap, a domain that forms a part of the RNA exit channel and is also a likely target for λ phage antiterminator proteins Q and N, and for bacterial elongation factor NusA. However, in contrast to Q and N, gp39 does not depend on NusA or other auxiliary factors for its activity. To our knowledge, gp39 is the first characterized phage-encoded transcription factor that affects every step of the transcription cycle and suppresses transcription termination through its antipausing activity.
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