ArticleNucleic acids research2012
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Redox control of antibiotic biosynthesis.mBio · 2025Article
- SydR, a redox-sensing MarR-type regulator ofmBio · 2024Article
- MarR Family Transcriptional Regulators and Their Roles in Plant-Interacting Bacteria.Microorganisms · 2023Review
- THOR's Hammer: the Antibiotic Koreenceine Drives Gene Expression in a Model Microbial Community.mBio · 2022Article
- The ArsH Protein Product of theAntioxidants (Basel, Switzerland) · 2022Article
- A Major Facilitator Superfamily (MFS) Efflux Pump, SCO4121, from Streptomyces coelicolor with Roles in Multidrug Resistance and Oxidative Stress Tolerance and Its Regulation by a MarR Regulator.Applied and environmental microbiology · 2021Article
- Genetically encoded formaldehyde sensors inspired by a protein intra-helical crosslinking reaction.Nature communications · 2021Article
- Response of Pseudomonas aeruginosa to the Innate Immune System-Derived Oxidants Hypochlorous Acid and Hypothiocyanous Acid.Journal of bacteriology · 2020Article
- Roles of RcsA, an AhpD Family Protein, in Reactive Chlorine Stress Resistance and Virulence in Pseudomonas aeruginosa.Applied and environmental microbiology · 2020Article
- The MarR-Type Repressor MhqR Confers Quinone and Antimicrobial Resistance inAntioxidants & redox signaling · 2019Article
- The Disulfide Stress Response and ProteinAntioxidants (Basel, Switzerland) · 2019Article
- An EmrB multidrug efflux pump inThe Journal of biological chemistry · 2019Article
- Redox-Sensing Under Hypochlorite Stress and Infection Conditions by the Rrf2-Family Repressor HypR in Staphylococcus aureus.Antioxidants & redox signaling · 2018Article
- RitR is an archetype for a novel family of redox sensors in the streptococci that has evolved from two-component response regulators and is required for pneumococcal colonization.PLoS pathogens · 2018Article
- A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.Scientific reports · 2017Article
- Metallochaperones and metalloregulation in bacteria.Essays in biochemistry · 2017Review
- Redox-Sensitive MarR Homologue BifR from Burkholderia thailandensis Regulates Biofilm Formation.Biochemistry · 2017Article
- The Oxidative Stress Agent Hypochlorite Stimulates c-di-GMP Synthesis and Biofilm Formation inFrontiers in microbiology · 2017Article
- Two distinct mechanisms of transcriptional regulation by the redox sensor YodB.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The activity of CouR, a MarR family transcriptional regulator, is modulated through a novel molecular mechanism.Nucleic acids research · 2016Article
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Authors and funding
9 authors.
Funding
Abstract
Bacillus subtilis encodes redox-sensing MarR-type regulators of the OhrR and DUF24-families that sense organic hydroperoxides, diamide, quinones or aldehydes via thiol-based redox-switches. In this article, we characterize the novel redox-sensing MarR/DUF24-family regulator HypR (YybR) that is activated by disulphide stress caused by diamide and NaOCl in B. subtilis. HypR controls positively a flavin oxidoreductase HypO that confers protection against NaOCl stress. The conserved N-terminal Cys14 residue of HypR has a lower pK(a) of 6.36 and is essential for activation of hypO transcription by disulphide stress. HypR resembles a 2-Cys-type regulator that is activated by Cys14-Cys49' intersubunit disulphide formation. The crystal structures of reduced and oxidized HypR proteins were resolved revealing structural changes of HypR upon oxidation. In reduced HypR a hydrogen-bonding network stabilizes the reactive Cys14 thiolate that is 8-9 Å apart from Cys49'. HypR oxidation breaks these H-bonds, reorients the monomers and moves the major groove recognition α4 and α4' helices ∼4 Å towards each other. This is the first crystal structure of a redox-sensing MarR/DUF24 family protein in bacteria that is activated by NaOCl stress. Since hypochloric acid is released by activated macrophages, related HypR-like regulators could function to protect pathogens against the host immune defense.
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