ArticleMolecular biology of the cell2012
Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 95 citations in OpenAlex.
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol.eLife · 2025Article
- Cooperative Function of Atg8- and TORC1-Mediated Activities in Yeast.Yeast (Chichester, England) · 2025Article
- Assembly and fission of tubular carriers mediating protein sorting in endosomes.Nature reviews. Molecular cell biology · 2024Review
- Automated quantification of vacuole fusion and lipophagy inAutophagy · 2024Article
- Article
- On the sampling strategies and models for measuring diffusion exchange with a double diffusion encoding sequence.Magnetic resonance letters · 2023Article
- Retromer oligomerization drives SNX-BAR coat assembly and membrane constriction.The EMBO journal · 2023Article
- The Rheb GTPase promotes pheromone blindness via a TORC1-independent pathway in the phytopathogenic fungus Ustilago maydis.PLoS genetics · 2022Article
- CROP: a retromer-PROPPIN complex mediating membrane fission in the endo-lysosomal system.The EMBO journal · 2022Article
- Robust Cre recombinase activity in the biotrophic smut fungus Ustilago maydis enables efficient conditional null mutants in planta.Genetics · 2022Article
- Acetate and hypertonic stress stimulate vacuole membrane fission using distinct mechanisms.PloS one · 2022Article
- WIPI1 promotes fission of endosomal transport carriers and formation of autophagosomes through distinct mechanisms.Autophagy · 2021Article
- SEA and GATOR 10 Years Later.Cells · 2021Review
- Early onset effects of single substrate accumulation recapitulate major features of LSD in patient-derived lysosomes.iScience · 2021Article
- Oxidative Stress Causes Vacuolar Fragmentation in the Human Fungal PathogenJournal of fungi (Basel, Switzerland) · 2021Article
- Subcellular Alterations Induced by Cyanotoxins in Vascular Plants-A Review.Plants (Basel, Switzerland) · 2021Review
- The Protein Phosphatase PP2A Plays Multiple Roles in Plant Development by Regulation of Vesicle Traffic-Facts and Questions.International journal of molecular sciences · 2021Review
- Article
- Lysosomal size matters.Traffic (Copenhagen, Denmark) · 2020Review
- Homeostasis of branched-chain amino acids is critical for the activity of TOR signaling ineLife · 2019Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Size and copy number of organelles are influenced by an equilibrium of membrane fusion and fission. We studied this equilibrium on vacuoles-the lysosomes of yeast. Vacuole fusion can readily be reconstituted and quantified in vitro, but it had not been possible to study fission of the organelle in a similar way. Here we present a cell-free system that reconstitutes fragmentation of purified yeast vacuoles (lysosomes) into smaller vesicles. Fragmentation in vitro reproduces physiological aspects. It requires the dynamin-like GTPase Vps1p, V-ATPase pump activity, cytosolic proteins, and ATP and GTP hydrolysis. We used the in vitro system to show that the vacuole-associated TOR complex 1 (TORC1) stimulates vacuole fragmentation but not the opposing reaction of vacuole fusion. Under nutrient restriction, TORC1 is inactivated, and the continuing fusion activity then dominates the fusion/fission equilibrium, decreasing the copy number and increasing the volume of the vacuolar compartment. This result can explain why nutrient restriction not only induces autophagy and a massive buildup of vacuolar/lysosomal hydrolases, but also leads to a concomitant increase in volume of the vacuolar compartment by coalescence of the organelles into a single large compartment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.