ArticleJournal of virology2012
The Simian virus 40 late viral protein VP4 disrupts the nuclear envelope for viral release.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- Recent advances in discovery and functional analysis of the small proteins and microRNA expressed by polyomaviruses.Virology · 2025Review
- Nuclear envelope budding and its cellular functions.Nucleus (Austin, Tex.) · 2023Article
- Article
- Does the Evidence Support the Existence of the Simian Polyomavirus SV40 Vp4 Viroporin?mSphere · 2020Article
- Expression of novel proteins by polyomaviruses and recent advances in the structural and functional features of agnoprotein of JC virus, BK virus, and simian virus 40.Journal of cellular physiology · 2019Review
- Human polyomaviruses and cancer: an overview.Clinics (Sao Paulo, Brazil) · 2018Review
- The Presumed Polyomavirus Viroporin VP4 of Simian Virus 40 or Human BK Polyomavirus Is Not Required for Viral Progeny Release.Journal of virology · 2016Article
- Review
- Review
- Identification of Two Novel Members of the Tentative Genus Wukipolyomavirus in Wild Rodents.PloS one · 2015Article
- TMTC1 and TMTC2 are novel endoplasmic reticulum tetratricopeptide repeat-containing adapter proteins involved in calcium homeostasis.The Journal of biological chemistry · 2014Article
- Viroporins customize host cells for efficient viral propagation.DNA and cell biology · 2013Review
- The miRNA world of polyomaviruses.Virology journal · 2013Review
- SV40 late protein VP4 forms toroidal pores to disrupt membranes for viral release.Biochemistry · 2013Article
- A cornucopia of human polyomaviruses.Nature reviews. Microbiology · 2013Review
- The viroporin activity of the minor structural proteins VP2 and VP3 is required for SV40 propagation.The Journal of biological chemistry · 2013Article
- Design stars: how small DNA viruses remodel the host nucleus.Future virology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Simian virus 40 (SV40) appears to initiate cell lysis by expressing the late viral protein VP4 at the end of infection to aid in virus dissemination. To investigate the contribution of VP4 to cell lysis, VP4 was expressed in mammalian cells where it was predominantly observed along the nuclear periphery. The integrity of the nuclear envelope was compromised in these cells, resulting in the mislocalization of a soluble nuclear marker. Using assays that involved the cellular expression of VP4 or the treatment of cells with purified VP4, we found that the central hydrophobic domain and a proximal C-terminal nuclear localization signal of VP4 were required for (i) cytolysis associated with prolonged expression; (ii) nuclear envelope accumulation; and (iii) disruption of the nuclear, red blood cell, or host cell membranes. Furthermore, a conserved proline within the hydrophobic domain was required for membrane perforation, suggesting that this residue was crucial for VP4 cytolytic activity. These results indicate that VP4 forms pores in the nuclear membrane leading to lysis and virus release.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.