ArticleJournal of virology2012
Deep sequencing identifies viral and wasp genes with potential roles in replication of Microplitis demolitor Bracovirus.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
45 citing papers in PubMed, 80 citations in OpenAlex.
- A chromosome-scale genome assembly and functional studies implicate the viral RNA polymerase in transcription of most Microplitis demolitor bracovirus late genes.Journal of virology · 2026Article
- Systematic profiling of nudivirus-like genes reveals conserved and differentiated roles in a domesticated endogenous virus.PLoS pathogens · 2026Article
- Progress in the molecular phylogeny of Cotesia acuminata and C. melitaearum cryptic species complexes.PloS one · 2026Article
- Transcriptional dynamics during Heliothis zea nudivirus 1 infection in an ovarian cell line fromThe Journal of general virology · 2025Article
- Functional characterization ofJournal of virology · 2023Article
- Conserved Viral Transcription Plays a Key Role in Virus-Like Particle Production of the Parasitoid WaspJournal of virology · 2022Article
- The Complete Genome ofJournal of virology · 2022Article
- Bracovirus Sneaks Into Apoptotic Bodies Transmitting Immunosuppressive Signaling Driven by Integration-Mediated eIF5A Hypusination.Frontiers in immunology · 2022Article
- Article
- MdBVe46 is an envelope protein that is required for virion formation by Microplitis demolitor bracovirus.The Journal of general virology · 2021Article
- Chromosomal scale assembly of parasitic wasp genome reveals symbiotic virus colonization.Communications biology · 2021Article
- MicroRNAs from Snellenius manilae bracovirus regulate innate and cellular immune responses of its host Spodoptera litura.Communications biology · 2021Article
- Lipid Dynamics, Identification, and Expression Patterns of Fatty Acid Synthase Genes in an Endoparasitoid,International journal of molecular sciences · 2020Article
- Genomic architecture of endogenous ichnoviruses reveals distinct evolutionary pathways leading to virus domestication in parasitic wasps.BMC biology · 2020Article
- Whole Genome Sequence of the Parasitoid WaspG3 (Bethesda, Md.) · 2018Article
- Persistent expression of Cotesia plutellae bracovirus genes in parasitized host, Plutella xylostella.PloS one · 2018Article
- A Horizontally Transferred Autonomous Helitron Became a Full Polydnavirus Segment inG3 (Bethesda, Md.) · 2017Article
- Review
- Expression differences inPeerJ · 2017Article
- Translational Control of Host Gene Expression by a Cys-Motif Protein Encoded in a Bracovirus.PloS one · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Viruses in the genus Bracovirus (BV) (Polydnaviridae) are symbionts of parasitoid wasps that specifically replicate in the ovaries of females. Recent analysis of expressed sequence tags from two wasp species, Cotesia congregata and Chelonus inanitus, identified transcripts related to 24 different nudivirus genes. These results together with other data strongly indicate that BVs evolved from a nudivirus ancestor. However, it remains unclear whether BV-carrying wasps contain other nudivirus-like genes and what types of wasp genes may also be required for BV replication. Microplitis demolitor carries Microplitis demolitor bracovirus (MdBV). Here we characterized MdBV replication and performed massively parallel sequencing of M. demolitor ovary transcripts. Our results indicated that MdBV replication begins in stage 2 pupae and continues in adults. Analysis of prereplication- and active-replication-stage ovary RNAs yielded 22 Gb of sequence that assembled into 66,425 transcripts. This breadth of sampling indicated that a large percentage of genes in the M. demolitor genome were sequenced. A total of 41 nudivirus-like transcripts were identified, of which a majority were highly expressed during MdBV replication. Our results also identified a suite of wasp genes that were highly expressed during MdBV replication. Among these products were several transcripts with conserved roles in regulating locus-specific DNA amplification by eukaryotes. Overall, our data set together with prior results likely identify the majority of nudivirus-related genes that are transcriptionally functional during BV replication. Our results also suggest that amplification of proviral DNAs for packaging into BV virions may depend upon the replication machinery of wasps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.