ArticleJournal of the American Society for Mass Spectrometry2012
A new approach to high sensitivity liquid chromatography-mass spectrometry of peptides using nanoflow solvent assisted inlet ionization.
Article in Journal of the American Society for Mass Spectrometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- New Processes for Ionizing Nonvolatile Compounds in Mass Spectrometry: The Road of Discovery to Current State-of-the-Art.Journal of the American Society for Mass Spectrometry · 2024Review
- Characterization of Complex Proteoform Mixtures by Online Nanoflow Ion-Exchange Chromatography-Native Mass Spectrometry.Analytical chemistry · 2024Article
- Direct sub-atmospheric pressure ionization mass spectrometry: Evaporation/sublimation-driven ionization is amazing, fundamentally, and practically.Journal of mass spectrometry : JMS · 2024Review
- Combining Field-Enabled Capillary Vibrating Sharp-Edge Spray Ionization with Microflow Liquid Chromatography and Mass Spectrometry to Enhance 'Omics Analyses.Journal of the American Society for Mass Spectrometry · 2021Article
- Improving the Sensitivity of Matrix-Assisted Ionization (MAI) Mass Spectrometry Using Ammonium Salts.Journal of the American Society for Mass Spectrometry · 2015Article
- On the ionization and ion transmission efficiencies of different ESI-MS interfaces.Journal of the American Society for Mass Spectrometry · 2015Article
- Drug detection and quantification directly from tissue using novel ionization methods for mass spectrometry.European journal of mass spectrometry (Chichester, England) · 2015Article
- Improving the sensitivity of mass spectrometry by using a new sheath flow electrospray emitter array at subambient pressures.Journal of the American Society for Mass Spectrometry · 2014Article
- Qualitative and quantitative mass spectrometry imaging of drugs and metabolites.Advanced drug delivery reviews · 2013Review
- Matrix assisted ionization in vacuum, a sensitive and widely applicable ionization method for mass spectrometry.Journal of the American Society for Mass Spectrometry · 2013Article
- Protein analysis by shotgun/bottom-up proteomics.Chemical reviews · 2013Review
- Carbonation and other super saturated gases as solution modifiers for improved sensitivity in solvent assisted ionization inlet (SAII) and ESI.Journal of the American Society for Mass Spectrometry · 2013Article
- Matrix assisted ionization: new aromatic and nonaromatic matrix compounds producing multiply charged lipid, peptide, and protein ions in the positive and negative mode observed directly from surfaces.Journal of the American Society for Mass Spectrometry · 2012Article
- A mechanism for ionization of nonvolatile compounds in mass spectrometry: considerations from MALDI and inlet ionization.Journal of the American Society for Mass Spectrometry · 2012Article
- Localization and imaging of gangliosides in mouse brain tissue sections by laserspray ionization inlet.Journal of lipid research · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liquid chromatography (LC) solvent assisted inlet ionization (SAII) mass spectrometry (MS) was previously reported to give good chromatographic resolution and MS detection injecting 66 ng of a BSA tryptic digest. In analogy to nano-electrospray ionization (nESI), we extend SAII LC/MS to nano-SAII (nSAII) operating at nL min(-1) flow rates and demonstrate good quality ion chromatograms and mass spectra from injection of as little as 0.7 ng of BSA digest onto a capillary LC column. Data dependent fragmentation is demonstrated for injection of 7 ng of a BSA digest. This method has advantages over nESI in ease of use and low cost as it requires no voltage and is operational without the necessity of connectors or fragile nESI emitters, although similar constricted tips can be helpful in nSAII to stabilize the signal at low nanoliter flow. At a flow rate of 0.8 μL min(-1), the only requirement for nSAII is that the exit-end of the capillary LC column be adjusted near the aperture of the heated inlet of the mass spectrometer.
Indexed as
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.