ArticleClinical and vaccine immunology : CVI2012
Development of a bead-based multiplex immunoassay for simultaneous quantitative detection of IgG serum antibodies against measles, mumps, rubella, and varicella-zoster virus.
Article in Clinical and vaccine immunology : CVI, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
58 citing papers in PubMed.
- Parenteral Vaccine-Induced Antibody Levels in Children with Heavy Antibiotic Exposure in Dhaka, Bangladesh.The American journal of tropical medicine and hygiene · 2026Trial
- Immunogenicity and safety of a measles and rubella-containing vaccine at age 6 and 9 months in Bangladesh: an open-label, randomised trial.The Lancet. Child & adolescent health · 2025Trial
- Iron Deficiency Anemia at Time of Vaccination Predicts Decreased Vaccine Response and Iron Supplementation at Time of Vaccination Increases Humoral Vaccine Response: A Birth Cohort Study and a Randomized Trial Follow-Up Study in Kenyan Infants.Frontiers in immunology · 2020Trial
- Seroprevalence of mumps in The Netherlands: dynamics over a decade with high vaccination coverage and recent outbreaks.PloS one · 2013Trial
- A multiplex serological assay for quantification of arbovirus antibody kinetics and neutralizing antibodies.PLoS neglected tropical diseases · 2026Article
- High-dimensional profiling following third MMR vaccination or mumps virus infection reveals individuals with suboptimal immunity.Communications medicine · 2026Article
- Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance.Scientific reports · 2026Article
- Multi-pathogen serosurveillance in Tonga examining demographic correlates and geographic clustering of 17 pathogens.Scientific reports · 2026Article
- Insights into the heterogeneity of pneumococcal polysaccharide vaccine immunogenicity in older adults.Immunity & ageing : I & A · 2026Article
- Antibody waning and Bordetella pertussis resurgence after the COVID-19 pandemic in the Netherlands.Nature communications · 2026Article
- Trends and patterns of varicella zoster virus infection in Kilifi: A population-based serosurvey.PLOS global public health · 2026Article
- Modelling the relative contribution of infection, routine vaccination and supplementary immunisation activities to measles seroconversion in Kenyan Children.PLoS computational biology · 2025Article
- Multiplex bead assays enable integrated serological surveillance and reveal cross-pathogen vulnerabilities in Zambezia Province, Mozambique.Nature communications · 2025Article
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- Article
- Serodynamics: A primer and synthetic review of methods for epidemiological inference using serological data.Epidemics · 2024Review
- The Global Measles and Rubella Laboratory Network Supports High-Quality Surveillance.Vaccines · 2024Article
- Serosurveillance for Measles and Rubella.Vaccines · 2024Review
- High-resolution African HLA resource uncovers HLA-DRB1 expression effects underlying vaccine response.Nature medicine · 2024Article
- shinyMBA: a novel R shiny application for quality control of the multiplex bead assay for serosurveillance studies.Scientific reports · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enzyme-linked immunosorbent assay (ELISA) is normally used to quantify the amount of serum IgG antibodies against measles, mumps, rubella, and varicella-zoster virus (MMRV). However, this method is time- and material-consuming. Therefore, a multiplex immunoassay for the simultaneous quantitative detection of antibodies against MMRV was developed. In-house as well as commercially available antigens can be used, making the assay available for all laboratories. The multiplex assay is much more sensitive than the separate ELISAs and has a high specificity, and only 5 μl of serum is needed. Heterologous inhibition did not exceed 11.5%, while homologous inhibition varied between 91.3 and 97.9%. Good correlations with the in-house ELISAs for measles (R(2) = 0.98), mumps (R(2) = 0.97), and rubella (R(2) = 0.97) virus as well as with the ELISA kit for varicella-zoster virus (R(2) = 0.95) were obtained. In conclusion, the MMRV multiplex assay is a good alternative to the conventional ELISAs and suitable for use in serosurveillance and vaccine studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.