ArticleThe Journal of allergy and clinical immunology2012
Usefulness of PBMCs to predict clinical response to corticosteroids in asthmatic patients.
Article in The Journal of allergy and clinical immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Association between glucocorticoid receptor beta and steroid resistance: A systematic review.Immunity, inflammation and disease · 2024Pooled it
- Single-Cell Transcriptomic Profiling Reveals a Distinct Monocyte MIF/ANXA1 Signature Associated With Poor Responsiveness to ICS.Allergy, asthma & immunology research · 2025Article
- Perinatal glucocorticoid sensitivity in the preterm newborn: molecular mechanisms, endogenous determinants, and clinical implications.Frontiers in endocrinology · 2025Review
- IL-10 and IL-6/IL-10 as predictive biomarkers for treatment response in non-infectious uveitis.Frontiers in immunology · 2025Article
- The Glucocorticoid Receptor: Isoforms, Functions, and Contribution to Glucocorticoid Sensitivity.Endocrine reviews · 2024Review
- Reference Gene Validation for RT-qPCR in PBMCs from Asthmatic Patients with or without Obesity.Methods and protocols · 2022Article
- Potential Role of Mast Cells in Regulating Corticosteroid Insensitivity in Severe Asthma.Advances in experimental medicine and biology · 2021Article
- Pharmacological Rationale for Targeting IL-17 in Asthma.Frontiers in allergy · 2021Review
- Different Biological Pathways Between Good and Poor Inhaled Corticosteroid Responses in Asthma.Frontiers in medicine · 2021Article
- Genetic Signatures of Acute Asthma Exacerbation Related With Ineffective Response to Corticosteroid.Allergy, asthma & immunology research · 2020Article
- miR-146a in Myasthenia Gravis Thymus Bridges Innate Immunity With Autoimmunity and Is Linked to Therapeutic Effects of Corticosteroids.Frontiers in immunology · 2020Article
- Characterization of MicroRNA and Gene Expression Profiles Following Ricin Intoxication.Toxins · 2019Article
- Expression of corticosteroid-regulated genes by PBMCs in children with asthma.The Journal of allergy and clinical immunology · 2019Article
- Effects of Glucocorticoid-Induced Transcript 1 Gene Deficiency on Glucocorticoid Activation in Asthmatic Mice.Chinese medical journal · 2018Article
- Glucocorticoid receptor isoform expression in peripheral blood mononuclear leukocytes of patients with chronic rhinosinusitis.International forum of allergy & rhinology · 2018Article
- Different Gene Expressions of Alpha and Beta Glucocorticoid Receptors in Asthmatics.Iranian journal of pharmaceutical research : IJPR · 2018Article
- Impaired anti-inflammatory action of glucocorticoid in neutrophil from patients with steroid-resistant asthma.Respiratory research · 2016Article
- Cysteine oxidation impairs systemic glucocorticoid responsiveness in children with difficult-to-treat asthma.The Journal of allergy and clinical immunology · 2015Article
- Characterization of a high TNF-α phenotype in children with moderate-to-severe asthma.The Journal of allergy and clinical immunology · 2015Article
- Peripheral blood IRF1 expression as a marker for glucocorticoid sensitivity.Pharmacogenetics and genomics · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundBlood tests are needed to identify steroid-resistant (SR) asthmatic patients early so that they can be managed with alternative anti-inflammatory therapy.
objectiveWe sought to assess the usefulness of peripheral blood to predict steroid response in asthmatic patients.
methodsNineteen asthmatic patients with FEV(1) of less than 80% of predicted value were classified as SR or steroid sensitive (SS) based on change in lung FEV(1) percentage after 7 days of oral prednisone. Blood was collected at baseline (visit 1) and 30 days after prednisone administration (visit 3). PBMCs were cultured for 4 hours with or without 10(-7) mol/L dexamethasone, and cellular response to dexamethasone was determined by using real-time PCR based on expression analysis of steroid-regulated genes. Suppression of PHA-induced T-cell proliferation by dexamethasone was assessed.
resultsPrednisone significantly improved FEV(1) percentages in SS asthmatic patients (mean ± SE: 17.5% ± 2.4%) but not SR asthmatic patients (0.8% ± 2.0%, P < .001). Before prednisone treatment, mitogen-induced kinase phosphatase 1 (P = .01) and IL-8 mRNA (P < .05) levels were significantly higher in PBMCs from SR asthmatic patients. TNF-α (P < .05) and IL-8 fold suppression by dexamethasone (P < .05) were significantly reduced in PBMCs from SR asthmatic patients. The expression of glucocorticoid receptor (GCR) β, but not GCR-α, was significantly increased in PBMCs of SR asthmatic patients (P = .01). The dexamethasone inhibitory concentration of 50% for PBMC proliferation was significantly higher for SR asthmatic patients (P < .05). These markers no longer differed between groups in PBMCs 30 days after prednisone administration. The composite score of assays at baseline before prednisone was significantly different between SR and SS asthmatic patients (P < .001).
conclusionsPBMCs from SR asthmatic patients have higher baseline mitogen-induced kinase phosphatase 1, IL-8, and GCR-β mRNA levels; have a lower GCR-α/GCR-β mRNA ratio; are less responsive to suppression of TNF-α and IL-8 by dexamethasone; and require more dexamethasone to suppress T-cell proliferation compared with SS asthmatic patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.