ArticleStem cells and development2012
A molecular signature for purified definitive endoderm guides differentiation and isolation of endoderm from mouse and human embryonic stem cells.
Article in Stem cells and development, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- Enhancer remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm.Developmental cell · 2025Article
- Differentiation properties of 3D scaffolds nanostructured with multi-walled carbon nanotubes on human induced pluripotent stem cells.RSC advances · 2025Article
- The human glucocorticoid receptor variant rs6190 increases blood cholesterol and promotes atherosclerosis.The Journal of clinical investigation · 2025Article
- Updated single cell reference atlas for the starlet anemone Nematostella vectensis.Frontiers in zoology · 2024Article
- Canine induced pluripotent stem cells efficiently differentiate into definitive endoderm in 3D cell culture conditions using high-dose activin A.Regenerative therapy · 2022Article
- Human-Based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State of the Art with a Feto-Placental Organ-on-Chip Platform.International journal of environmental research and public health · 2022Review
- Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids.Development (Cambridge, England) · 2022Article
- Profiling of mature-stage human breast milk cells identifies six unique lactocyte subpopulations.Science advances · 2022Article
- A Bayesian factorization method to recover single-cell RNA sequencing data.Cell reports methods · 2022Article
- Article
- Epithelial cell plasticity drives endoderm formation during gastrulation.Nature cell biology · 2021Article
- Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells.Stem cells international · 2021Article
- Hepatogenic Potential and Liver Regeneration Effect of Human Liver-derived Mesenchymal-Like Stem Cells.Cells · 2020Article
- Application of iPSC to Modelling of Respiratory Diseases.Advances in experimental medicine and biology · 2020Review
- Fine Tuning of Hepatocyte Differentiation from Human Embryonic Stem Cells: Growth FactorStem cells international · 2019Article
- Multi-axial self-organization properties of mouse embryonic stem cells into gastruloids.Nature · 2018Article
- An accurate and robust imputation method scImpute for single-cell RNA-seq data.Nature communications · 2018Article
- Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells.Stem cell reports · 2018Article
- GATA6 is essential for endoderm formation from human pluripotent stem cells.Biology open · 2017Article
- Defined three-dimensional culture conditions mediate efficient induction of definitive endoderm lineage from human umbilical cord Wharton's jelly mesenchymal stem cells.Stem cell research & therapy · 2016Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Embryonic definitive endoderm (DE) generates the epithelial compartment of vital organs such as liver, pancreas, and intestine. However, purification of DE in mammals has not been achieved, limiting the molecular "definition" of endoderm, and hindering our understanding of DE development and attempts to produce endoderm from sources such as embryonic stem (ES) cells. Here, we describe purification of mouse DE using fluorescence-activated cell sorting (FACS) and mice harboring a transgene encoding enhanced green fluorescent protein (eGFP) inserted into the Sox17 locus, which is expressed in the embryonic endoderm. Comparison of patterns of signaling pathway activation in native mouse DE and endoderm-like cells generated from ES cells produced novel culture modifications that generated Sox17-eGFP⁺ progeny whose gene expression resembled DE more closely than achieved with standard methods. These studies also produced new FACS methods for purifying DE from nontransgenic mice and mouse ES cell cultures. Parallel studies of a new human SOX17-eGFP ES cell line allowed analysis of endoderm differentiation in vitro, leading to culture modifications that enhanced expression of an endoderm-like signature. This work should accelerate our understanding of mechanisms regulating DE development in mice and humans, and guide further use of ES cells for tissue replacement.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.