ArticleAnalytical chemistry2012
Nanohole-based surface plasmon resonance instruments with improved spectral resolution quantify a broad range of antibody-ligand binding kinetics.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 103 citations in OpenAlex.
- Fabrication of Metal-Insulator-Metal Nanostructures Composed of Au-MgFMicromachines · 2022Article
- Modulating Optical Characteristics of Nanoimprinted Plasmonic Device by Re-Shaping Process of Polymer Mold.Micromachines · 2021Article
- Fibre-Optic Surface Plasmon Resonance Biosensor for Monoclonal Antibody Titer Quantification.Biosensors · 2021Article
- A comprehensive review on plasmonic-based biosensors used in viral diagnostics.Communications biology · 2021Review
- Fabrication and Characterization of a Metallic-Dielectric Nanorod Array by Nanosphere Lithography for Plasmonic Sensing Application.Nanomaterials (Basel, Switzerland) · 2019Article
- Nanohole array plasmonic biosensors: Emerging point-of-care applications.Biosensors & bioelectronics · 2019Review
- Recent Advances in Surface Plasmon Resonance Imaging Sensors.Sensors (Basel, Switzerland) · 2019Review
- Rectangular plasmonic interferometer for high sensitive glycerol sensor.Scientific reports · 2019Article
- Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum.Journal of visualized experiments : JoVE · 2017Article
- SPR and SPR Imaging: Recent Trends in Developing Nanodevices for Detection and Real-Time Monitoring of Biomolecular Events.Sensors (Basel, Switzerland) · 2016Review
- Study of flow rate induced measurement error in flow-through nano-hole plasmonic sensor.Biomicrofluidics · 2015Article
- Plasmofluidics: Merging Light and Fluids at the Micro-/Nanoscale.Small (Weinheim an der Bergstrasse, Germany) · 2015Review
- Nanostar Clustering Improves the Sensitivity of Plasmonic Assays.Bioconjugate chemistry · 2015Article
- Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches.Sensors (Basel, Switzerland) · 2015Review
- Nano-plasmonic exosome diagnostics.Expert review of molecular diagnostics · 2015Review
- Plasmon-enhanced optical sensors: a review.The Analyst · 2015Review
- Quantification of a cardiac biomarker in human serum using extraordinary optical transmission (EOT).PloS one · 2015Article
- Advances in plasmonic technologies for point of care applications.Chemical reviews · 2014Review
- Applications of SPR for the characterization of molecules important in the pathogenesis and treatment of neurodegenerative diseases.Expert review of neurotherapeutics · 2014Review
- Nanopore-induced spontaneous concentration for optofluidic sensing and particle assembly.Analytical chemistry · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
We demonstrate an affordable low-noise surface plasmon resonance (SPR) instrument based on extraordinary optical transmission (EOT) in metallic nanohole arrays and quantify a broad range of antibody-ligand binding kinetics with equilibrium dissociation constants ranging from 200 pM to 40 nM. This nanohole-based SPR instrument is straightforward to construct, align, and operate, since it is built around a standard microscope and a portable fiber-optic spectrometer. The measured refractive index resolution of this platform is 3.1 × 10(-6) without on-chip cooling, which is among the lowest reported for SPR sensors based on EOT. This is accomplished via rapid full-spectrum acquisition in 10 ms followed by frame averaging of the EOT spectra, which is made possible by the production of template-stripped gold nanohole arrays with homogeneous optical properties over centimeter-sized areas. Sequential SPR measurements are performed using a 12-channel microfluidic flow cell after optimizing surface modification protocols and antibody injection conditions to minimize mass-transport artifacts. The immobilization of a model ligand, the protective antigen of anthrax on the gold surface, is monitored in real-time with a signal-to-noise ratio of ~860. Subsequently, real-time binding kinetic curves were measured quantitatively between the antigen and a panel of small, 25 kDa single-chain antibodies at concentrations down to 1 nM. These results indicate that nanohole-based SPR instruments have potential for quantitative antibody screening and as a general-purpose platform for integrating SPR sensors with other bioanalytical tools.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.