ArticlePLoS neglected tropical diseases2012
The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 1 synthesis or guideline pooled it, 105 citations in OpenAlex.
- Whole Genome Sequencing and Comparative Genomics of the Emerging PathogenPathogens (Basel, Switzerland) · 2025Pooled it
- Mucosal-associated invariant T (MAIT) cells are reduced and dysfunctional in acute melioidosis.Scientific reports · 2026Article
- Concurrent resistance and virulence traits in extremely drug-resistant Burkholderia pseudomallei from bovine milk samples: potential for zoonotic transmission.Veterinary research communications · 2026Article
- Laboratory diagnosis of melioidosis.PLoS neglected tropical diseases · 2025Review
- Multisystemic Disease and Septicemia Caused by Presumptive Burkholderia pseudomallei in American Quarter Horse, Florida, USA.Emerging infectious diseases · 2025Article
- Virulence and genome analysis of three historicalFrontiers in cellular and infection microbiology · 2025Article
- Molecular epidemiology ofInfectious medicine · 2024Article
- Performance of Antibody-Detection Tests for Human Melioidosis: A Systematic Review and Meta-analysis.The Malaysian journal of medical sciences : MJMS · 2024Review
- A molecular epidemiological analysis of Burkholderia pseudomallei in southern Thailand.PLoS neglected tropical diseases · 2024Article
- The Genomic Epidemiology of ClinicalPathogens (Basel, Switzerland) · 2024Article
- Diversity, Complexity, and Specificity of Bacterial Lipopolysaccharide (LPS) Structures Impacting Their Detection and Quantification.International journal of molecular sciences · 2024Article
- Review
- TetR-like regulator BP1026B_II1561 controls aromatic amino acid biosynthesis and intracellular pathogenesis inFrontiers in microbiology · 2024Article
- The Arabinose 5-Phosphate Isomerase KdsD Is Required for Virulence in Burkholderia pseudomallei.Journal of bacteriology · 2023Article
- Variable Virulence Genes in Clinical Isolates ofJournal of laboratory physicians · 2023Article
- Pathogenicity and virulence ofVirulence · 2022Review
- Cycle-Inhibiting Factor Is Associated withBiology · 2022Article
- Recipient Cell Factors Influence Interbacterial Competition Mediated by Two Distinct Burkholderia dolosa Contact-Dependent Growth Inhibition Systems.Journal of bacteriology · 2022Article
- Article
- Biogeography and genetic diversity of clinical isolates of Burkholderia pseudomallei in Sri Lanka.PLoS neglected tropical diseases · 2021Article
3 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 3 institutions in 3 countries.
Funding
Abstract
Lipopolysaccharide (LPS) is one of the most important virulence and antigenic components of Burkholderia pseudomallei, the causative agent of melioidosis. LPS diversity in B. pseudomallei has been described as typical, atypical or rough, based upon banding patterns on SDS-PAGE. Here, we studied the genetic and molecular basis of these phenotypic differences. Bioinformatics was used to determine the diversity of genes known or predicted to be involved in biosynthesis of the O-antigenic moiety of LPS in B. pseudomallei and its near-relative species. Multiplex-PCR assays were developed to target diversity of the O-antigen biosynthesis gene patterns or LPS genotypes in B. pseudomallei populations. We found that the typical LPS genotype (LPS genotype A) was highly prevalent in strains from Thailand and other countries in Southeast Asia, whereas the atypical LPS genotype (LPS genotype B) was most often detected in Australian strains (~13.8%). In addition, we report a novel LPS ladder pattern, a derivative of the atypical LPS phenotype, associated with an uncommon O-antigen biosynthesis gene cluster that is found in only a small B. pseudomallei sub-population. This new LPS group was designated as genotype B2. We also report natural mutations in the O-antigen biosynthesis genes that potentially cause the rough LPS phenotype. We postulate that the diversity of LPS may correlate with differential immunopathogenicity and virulence among B. pseudomallei strains.
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Registered trials
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