ArticlePloS one2012
First description of natural and experimental conjugation between Mycobacteria mediated by a linear plasmid.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Widespread macrolide resistance among rapidly growing mycobacteria due to plasmids containing erm(55).Scientific reports · 2026Article
- Giant linear plasmids in Mycobacterium avium harbour a tRNA array unit.DNA research : an international journal for rapid publication of reports on genes and genomes · 2026Article
- Genomic insights into the plasmidome of non-tuberculous mycobacteria.Genome medicine · 2025Article
- Comparative genomics of Mycobacterium avium subsp. hominissuis strains within a group of captive lowland tapirs.PloS one · 2025Article
- Genomic diversity and clinical relevance ofERJ open research · 2024Article
- Emergence of Inducible Macrolide Resistance in Mycobacterium chelonae Due to Broad-Host-Range Plasmid and Chromosomal Variants of the Novel 23S rRNA Methylase Gene,Journal of clinical microbiology · 2023Article
- Genomic Epidemiological Analysis of Antimicrobial-Resistant Bacteria with Nanopore Sequencing.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Comparative genome analysis reveals high-level drug resistance markers in a clinical isolate ofFrontiers in cellular and infection microbiology · 2022Article
- Insights into the ancestry evolution of theNAR genomics and bioinformatics · 2021Article
- Pathogenic Determinants of theMicroorganisms · 2021Article
- Genomics of Atlantic ForestMicrobial genomics · 2020Article
- Comparative Genomic and Transcriptomic Analyses ofFrontiers in cellular and infection microbiology · 2020Article
- Global Assessment of Mycobacterium avium subsp.mSystems · 2019Article
- Circulation ofPathogens and global health · 2019Review
- Validation of a Real-Time PCR for the Detection ofFrontiers in veterinary science · 2019Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Mycobacterial Pan-Genome Analysis Suggests Important Role of Plasmids in the Radiation of Type VII Secretion Systems.Genome biology and evolution · 2016Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn a previous study, we detected the presence of a Mycobacterium avium species-specific insertion sequence, IS1245, in Mycobacterium kansasii. Both species were isolated from a mixed M. avium-M. kansasii bone marrow culture from an HIV-positive patient. The transfer mechanism of this insertion sequence to M. kansasii was investigated here. METHODOLOGY/PRINCIPAL
findingsA linear plasmid (pMA100) was identified in all colonies isolated from the M. avium-M. kansasii mixed culture carrying the IS1245 element. The linearity of pMA100 was confirmed. Other analyses suggested that pMA100 contained a covalently bound protein in the terminal regions, a characteristic of invertron linear replicons. Partial sequencing of pMA100 showed that it bears one intact copy of IS1245 inserted in a region rich in transposase-related sequences. These types of sequences have been described in other linear mycobacterial plasmids. Mating experiments were performed to confirm that pMA100 could be transferred in vitro from M. avium to M. kansasii. pMA100 was transferred by in vitro conjugation not only to the M. kansasii strain from the mixed culture, but also to two other unrelated M. kansasii clinical isolates, as well as to Mycobacterium bovis BCG Moreau. CONCLUSIONS/SIGNIFICANCE: Horizontal gene transfer (HGT) is one of most important mechanisms leading to the evolution and diversity of bacteria. This work provides evidence for the first time on the natural occurrence of HGT between different species of mycobacteria. Gene transfer, mediated by a novel conjugative plasmid, was detected and experimentally reproduced.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.