ArticleScience signaling2012
A dual array-based approach to assess the abundance and posttranslational modification state of signaling proteins.
Article in Science signaling, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
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- Polypharmacology-based kinome screen identifies new regulators of KSHV reactivation.bioRxiv : the preprint server for biology · 2023Article
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- Anesthetics inhibit phosphorylation of the ribosomal protein S6 in mouse cultured cortical cells and developing brain.Frontiers in aging neuroscience · 2023Article
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- Computational modeling identifies multitargeted kinase inhibitors as effective therapies for metastatic, castration-resistant prostate cancer.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Representing dynamic biological networks with multi-scale probabilistic models.Communications biology · 2019Article
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- Aberrantly activated Cox-2 and Wnt signaling interact to maintain cancer stem cells in glioblastoma.Oncotarget · 2017Article
- Bulk cell density and Wnt/TGFbeta signalling regulate mesendodermal patterning of human pluripotent stem cells.Nature communications · 2016Article
- Reverse Phase Protein Arrays for Compound Profiling.Current protocols in chemical biology · 2016Article
- A Nexus Consisting of Beta-Catenin and Stat3 Attenuates BRAF Inhibitor Efficacy and Mediates Acquired Resistance to Vemurafenib.EBioMedicine · 2016Article
- Co-clinical assessment identifies patterns of BRAF inhibitor resistance in melanoma.The Journal of clinical investigation · 2015Article
- Profiling phospho-signaling networks in breast cancer using reverse-phase protein arrays.Oncogene · 2013Article
- Suppression of host p53 is critical for Plasmodium liver-stage infection.Cell reports · 2013Article
Corrections and comments
- Erratum issuedSevecka, Mark [added]
Authors and funding
8 authors.
Funding
Abstract
A system-wide analysis of cell signaling requires detecting and quantifying many different proteins and their posttranslational modification states in the same cellular sample. Here, we present Protocols for two miniaturized, array-based methods, one of which provides detailed information on a central signaling protein and the other of which provides a broad characterization of the surrounding signaling network. We describe a bead-based array and its use in characterizing the different forms and functions of β-catenin, as well as lysate microarrays (reverse-phase protein arrays) and their use in detecting and quantifying proteins involved in the canonical and noncanonical Wnt signaling pathways. As an application of this dual approach, we characterized the state of β-catenin signaling in cell lysates and linked these molecule-specific data with pathway-wide changes in signaling. The Protocols described here provide detailed instructions for cell culture methods, bead arrays, and lysate microarrays and outline how to use these complementary approaches to obtain insight into a complex network at a systems level.
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