ArticlePlant cell reports2012
Highly efficient sorghum transformation.
Article in Plant cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- FUL1 is a key regulator of grain morphology, stem development, and other shoot characteristics inaBIOTECH · 2026Article
- Article
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- The PIN2 ortholog in barley modifies root gravitropism and architecture.The plant genome · 2025Article
- An accelerated transgene-free genome editing system using microparticle bombardment of sorghum immature embryos.aBIOTECH · 2025Article
- Sorghum as a monocot model for drought research.Frontiers in plant science · 2025Review
- Review
- New methods for sorghum transformation in temperate climates.AoB PLANTS · 2023Review
- Characterization of a foxtail mosaic virus vector for gene silencing and analysis of innate immune responses in Sorghum bicolor.Molecular plant pathology · 2023Article
- Recent advancements in the breeding of sorghum crop: current status and future strategies for marker-assisted breeding.Frontiers in genetics · 2023Review
- Article
- Genetic modification of PIN genes induces causal mechanisms of stay-green drought adaptation phenotype.Journal of experimental botany · 2022Article
- Highly Efficient and Reproducible Genetic Transformation in Pea for Targeted Trait Improvement.ACS agricultural science & technology · 2022Article
- Progress and challenges in sorghum biotechnology, a multipurpose feedstock for the bioeconomy.Journal of experimental botany · 2022Review
- Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum.The New phytologist · 2021Article
- Sorghum genetic, genomic, and breeding resources.Planta · 2021Review
- Review
- Over-Expression of Endogenous SUGARWIN Genes Exalted Tolerance againstPlants (Basel, Switzerland) · 2021Article
- Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum.Biotechnology for biofuels · 2021Article
- Current status and prospects of plant genome editing in Australia.In vitro cellular & developmental biology. Plant : journal of the Tissue Culture Association · 2021Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A highly efficient microprojectile transformation system for sorghum (Sorghum bicolor L.) has been developed by using immature embryos (IEs) of inbred line Tx430. Co-bombardment was performed with the neomycin phosphotransferase II (nptII) gene and the green fluorescent protein (gfp) gene, both under the control of the maize ubiquitin1 (ubi1) promoter. After optimization of both tissue culture media and parameters of microprojectile transformation, 25 independent transgenic events were obtained from 121 bombarded IEs. The average transformation frequency (the total number of independent transgenic events divided by the total number of bombarded IEs) was 20.7% in three independent experiments. Transgenic events were confirmed by both PCR screening and Southern hybridization of genomic DNA from primary transgenics (T₀). More than 90% of transformants were fertile and displayed normal morphology in a containment glasshouse. Co-transformation rate of the nptII and gfp genes was 72% in these experiments. The segregation of nptII and gfp in T₁ progenies was observed utilizing fluorescence microscopy and geneticin selection of seedlings indicating both were inherited in the T₁ generation. The transformation procedure, from initiating IEs to planting putative transgenic plantlets in the glasshouse, was completed within 11-16 weeks, and was approximately threefold more efficient than the previously reported best sorghum transformation system.
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