ArticleExperimental cell research2012
Corneal stromal cells use both high- and low-contractility migration mechanisms in 3-D collagen matrices.
Article in Experimental cell research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- The cell-fate decision of dental follicle stem cells (DFSCs) with porcine corneal extracellular matrix (ECM).Journal of dental sciences · 2025Article
- Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing.International journal of molecular sciences · 2022Article
- Unraveling the mechanobiology of cornea: From bench side to the clinic.Frontiers in bioengineering and biotechnology · 2022Review
- Keratocyte mechanobiology.Experimental eye research · 2020Review
- Improvement of chronic corneal opacity in ocular surface disease with prosthetic replacement of the ocular surface ecosystem (PROSE) treatment.American journal of ophthalmology case reports · 2018Article
- Fibroblast-fibronectin patterning and network formation in 3D fibrin matrices.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Corneal Decellularization: A Method of Recycling Unsuitable Donor Tissue for Clinical Translation?Current eye research · 2016Article
- Peptide Amphiphiles in Corneal Tissue Engineering.Journal of functional biomaterials · 2015Review
- Fibroblastic Transformation of Corneal Keratocytes by Rac Inhibition is Modulated by Extracellular Matrix Structure and Stiffness.Journal of functional biomaterials · 2015Article
- Mechanical interactions and crosstalk between corneal keratocytes and the extracellular matrix.Experimental eye research · 2015Review
- The Role of Thrombin and Cell Contractility in Regulating Clustering and Collective Migration of Corneal Fibroblasts in Different ECM Environments.Investigative ophthalmology & visual science · 2015Article
- Effect of HDAC Inhibitors on Corneal Keratocyte Mechanical Phenotypes in 3-D Collagen Matrices.Molecular vision · 2015Article
- Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ.PloS one · 2015Article
- MMP regulation of corneal keratocyte motility and mechanics in 3-D collagen matrices.Experimental eye research · 2014Article
- Corneal stromal cell plasticity: in vitro regulation of cell phenotype through cell-cell interactions in a three-dimensional model.Tissue engineering. Part A · 2014Article
- Techniques for assessing 3-D cell-matrix mechanical interactions in vitro and in vivo.Experimental cell research · 2013Review
- At the leading edge of three-dimensional cell migration.Journal of cell science · 2012Review
- Control of scar tissue formation in the cornea: strategies in clinical and corneal tissue engineering.Journal of functional biomaterials · 2012Article
- Individual versus collective fibroblast spreading and migration: regulation by matrix composition in 3D culture.Experimental eye research · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Corneal keratocyte migration can impact both corneal clarity and refractive outcome following injury or refractive surgery. In this study, we investigated how culture conditions, ECM properties, and Rho kinase activity regulate the mechanics of keratocyte migration, using a nested collagen matrix model. Time-lapse imaging demonstrated that both serum and PDGF stimulate keratocyte migration into the outer matrix. Although the velocity of cell migration was similar, cells in serum were bipolar and induced significant matrix deformation during migration, whereas PDGF induced extension of branching dendritic processes with smaller, more localized force generation. These differences in cell-induced matrix reorganization were verified with a global matrix contraction assay and confocal reflection imaging, using both bovine and rat tail collagen. When constructs were detached from the substrate to lower the effective stiffness, migration was significantly reduced in serum; but was unchanged in PDGF. These differences in migration mechanics were mediated, in part, by Rho kinase. Overall, corneal keratocytes can effectively migrate through collagen matrices using varying degrees of cellular force generation. Low-contractility migration may facilitate keratocyte repopulation of the stroma following surgery or injury, without altering the structural and mechanical properties that are critical to maintaining corneal transparency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.