ArticleDevelopmental biology2012
RFX2 is essential in the ciliated organ of asymmetry and an RFX2 transgene identifies a population of ciliated cells sufficient for fluid flow.
Article in Developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Serotonin has early, cilia-independent roles in Xenopus left-right patterning.Disease models & mechanisms · 2013Pooled it
- Primary cilia promote the differentiation of human neurons through the WNT signaling pathway.BMC biology · 2024Article
- Transcriptional Regulation of Airway Epithelial Cell Differentiation: Insights into the Notch Pathway and Beyond.International journal of molecular sciences · 2023Review
- GEMC1 and MCIDAS interactions with SWI/SNF complexes regulate the multiciliated cell-specific transcriptional program.Cell death & disease · 2023Article
- Molecular characteristics and transcriptional regulatory of spermatogenesis-related geneAnimal reproduction · 2023Article
- Article
- RFX1: a promising therapeutic arsenal against cancer.Cancer cell international · 2021Review
- The First Report of a Missense Variant inFrontiers in genetics · 2021Article
- Epithelial Barrier Dysfunction in Chronic Respiratory Diseases.Frontiers in physiology · 2021Review
- Interplay of RFX transcription factors 1, 2 and 3 in motile ciliogenesis.Nucleic acids research · 2020Article
- Article
- Histone H2B monoubiquitination regulates heart development via epigenetic control of cilia motility.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion.Scientific reports · 2018Article
- Zebrafish Rfx4 controls dorsal and ventral midline formation in the neural tube.Developmental dynamics : an official publication of the American Association of Anatomists · 2018Article
- Systematic target function annotation of human transcription factors.BMC biology · 2018Article
- Vertebrate Left-Right Asymmetry: What Can Nodal Cascade Gene Expression Patterns Tell Us?Journal of cardiovascular development and disease · 2017Review
- Dynamics of cilia length in left-right development.Royal Society open science · 2017Article
- Claudin5a is required for proper inflation of Kupffer's vesicle lumen and organ laterality.PloS one · 2017Article
- Ciliary dyslexia candidate genes DYX1C1 and DCDC2 are regulated by Regulatory Factor X (RFX) transcription factors through X-box promoter motifs.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2016Article
- Transcription Factor RFX2 Is a Key Regulator of Mouse Spermiogenesis.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Motile cilia create asymmetric fluid flow in the evolutionarily conserved ciliated organ of asymmetry (COA) and play a fundamental role in establishing the left-right (LR) axis in vertebrate embryos. The transcriptional control of the large group of genes that encode proteins that contribute to ciliary structure and function remains poorly understood. In this study we find that the winged helix transcription factor Rfx2 is expressed in motile cilia in mouse and zebrafish embryos. Morpholino knockdown of Rfx2 function in the whole embryo or specifically in cells of the zebrafish COA (Kupffer's Vesicle, KV) leads to reduced KV cilia length and perturbations in LR asymmetry. LR patterning defects include randomization of the early asymmetric Nodal signaling pathway genes southpaw, lefty1 and lefty2 and subsequent reversals in the organ primordia of the heart and gut. Rfx2 is also required for ciliogenesis in zebrafish pronephric duct. We further show that by restoring Left-Right dynein (LRD) expression and motility specifically in a subset of ciliated cells of the mouse COA (posterior notochord, PNC), we can restore fluid flow, asymmetric expression of Pitx2 and partially rescue situs defects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.