ArticleBMC genomics2012
Differential expression patterns of conserved miRNAs and isomiRs during Atlantic halibut development.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 87 citations in OpenAlex.
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- MiR-205 Mediated Cu-Induced Lipid Accumulation in Yellow CatfishInternational journal of molecular sciences · 2018Article
- MiR-202 controls female fecundity by regulating medaka oogenesis.PLoS genetics · 2018Article
- Circulating miRNA measurements are reflective of cholesterol-based changes in rainbow trout (Oncorhynchus mykiss).PloS one · 2018Article
- Differential miRNA expression in the three-spined stickleback, response to environmental changes.Scientific reports · 2017Article
- Identification and profiling of growth-related microRNAs in Chinese perch (Siniperca chuatsi).BMC genomics · 2017Article
- Evolution of Fish Let-7 MicroRNAs and Their Expression Correlated to Growth Development in Blunt Snout Bream.International journal of molecular sciences · 2017Article
- Dynamics of miRNA transcriptome during gonadal development of zebrafish.Scientific reports · 2017Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicroRNAs (miRNAs) play a major role in animal ontogenesis. Size variants of miRNAs, isomiRs, are observed along with the main miRNA types, but their origin and possible biological role are uncovered yet. Developmental profiles of miRNAs have been reported in few fish species only and, to our knowledge, differential expressions of isomiRs have not yet been shown during fish development. Atlantic halibut, Hippoglossus hippoglossus L., undergoes dramatic metamorphosis during early development from symmetrical pelagic larval stage to unsymmetrical flatfish. No data exist on role of miRNAs in halibut metamorphosis.
resultsmiRNA profiling using SOLiD deep sequencing technology revealed a total of 199 conserved, one novel antisense, and one miRNA* mature form. Digital expression profiles of selected miRNAs were validated using reverse transcription quantitative PCR. We found developmental transition-specific miRNA expression. Expression of some miRNA* exceeded the guide strand miRNA. We revealed that nucleotide truncations and/or additions at the 3' end of mature miRNAs resulted in size variants showing differential expression patterns during the development in a number of miRNA families. We confirmed the presence of isomiRs by cloning and Sanger sequencing. Also, we found inverse relationship between expression levels of sense/antisense miRNAs during halibut development.
conclusionDevelopmental transitions during early development of Atlantic halibut are associated with expression of certain miRNA types. IsomiRs are abundant and often show differential expression during the development.
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