ArticleProceedings of the National Academy of Sciences of the United States of America2012
Metabolic click-labeling with a fucose analog reveals pectin delivery, architecture, and dynamics in Arabidopsis cell walls.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 147 citations in OpenAlex.
- Fucoidans: a new frontier in brown seaweed research and biotechnology.Cell surface (Amsterdam, Netherlands) · 2026Review
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- Mechanical constraint causes lower turgor, thicker walls, and faster growth in Arabidopsis root hairs.Plant physiology · 2026Article
- Unlocking the black box: multimodal imaging and quantitative analysis of plant vesicular trafficking.Advanced biotechnology · 2026Review
- Genomic signatures inmSystems · 2026Article
- From Molecule to Meaning: Click and Bioorthogonal Chemical Reporters for Plant Systems, Biological Imaging, and Artistic Expression.Chembiochem : a European journal of chemical biology · 2026Review
- The epidermis coordinates multi-scale symmetry breaking in chiral root growth.Nature communications · 2025Article
- Click & sea: using bioorthogonal click chemistry to visualize seaweed cell walls.Annals of botany · 2025Article
- Storming the barricades of rhamnogalacturonan-II synthesis and function.The Plant cell · 2025Review
- Article
- Cell wall dynamics: novel tools and research questions.Journal of experimental botany · 2023Review
- The Light-Controlled Release of 2-fluoro-l-fucose, an Inhibitor of the Root Cell Elongation, from a nitrobenzyl-caged Derivative.International journal of molecular sciences · 2023Article
- Molecular sensors reveal the mechano-chemical response ofCell surface (Amsterdam, Netherlands) · 2022Article
- Arabidopsis root hairs do not only grow at their tip.Nature plants · 2022Article
- Shank-localized cell wall growth contributes to Arabidopsis root hair elongation.Nature plants · 2022Article
- Cell wall mechanics: Some new twists.Biophysical journal · 2022Article
- REPRISAL: mapping lignification dynamics using chemistry, data segmentation, and ratiometric analysis.Plant physiology · 2022Article
- Fifteen compelling open questions in plant cell biology.The Plant cell · 2022Review
- A glossary of plant cell structures: Current insights and future questions.The Plant cell · 2022Review
- Harnessing belowground processes for sustainable intensification of agricultural systems.Plant and soil · 2022Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polysaccharide-rich cell walls are a defining feature of plants that influence cell division and growth, but many details of cell-wall organization and dynamics are unknown because of a lack of suitable chemical probes. Metabolic labeling using sugar analogs compatible with click chemistry has the potential to provide new insights into cell-wall structure and dynamics. Using this approach, we found that an alkynylated fucose analog (FucAl) is metabolically incorporated into the cell walls of Arabidopsis thaliana roots and that a significant fraction of the incorporated FucAl is present in pectic rhamnogalacturonan-I (RG-I). Time-course experiments revealed that FucAl-containing RG-I first localizes in cell walls as uniformly distributed punctae that likely mark the sites of vesicle-mediated delivery of new polysaccharides to growing cell walls. In addition, we found that the pattern of incorporated FucAl differs markedly along the developmental gradient of the root. Using pulse-chase experiments, we also discovered that the pectin network is reoriented in elongating root epidermal cells. These results reveal previously undescribed details of polysaccharide delivery, organization, and dynamics in cell walls.
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