ArticleFrontiers in neuroenergetics2011
Critical state of energy metabolism in brain slices: the principal role of oxygen delivery and energy substrates in shaping neuronal activity.
Article in Frontiers in neuroenergetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 72 citations in OpenAlex.
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- Microglia contribute to neuronal synchrony despite endogenous ATP-related phenotypic transformation in acute mouse brain slices.Nature communications · 2024Article
- Herpes simplex virus infection induces necroptosis of neurons and astrocytes in human fetal organotypic brain slice cultures.Journal of neuroinflammation · 2024Article
- Time-dependent phenotypical changes of microglia drive alterations in hippocampal synaptic transmission in acute slices.Frontiers in cellular neuroscience · 2024Article
- Appraising the Role of Astrocytes as Suppliers of Neuronal Glutathione Precursors.International journal of molecular sciences · 2023Review
- Could respiration-driven blood oxygen changes modulate neural activity?Pflugers Archiv : European journal of physiology · 2023Review
- Assaying activity-dependent arteriole and capillary responses in brain slices.Neurophotonics · 2022Article
- Guidelines for the measurement of vascular function and structure in isolated arteries and veins.American journal of physiology. Heart and circulatory physiology · 2021Review
- Metabolic Changes in Brain Slices over Time: a Multiplatform Metabolomics Approach.Molecular neurobiology · 2021Article
- Electrochemical degradation of psychotropic pharmaceutical compounds from municipal wastewater and neurotoxicity evaluations.Environmental science and pollution research international · 2021Article
- Cerebrospinal fluid oxygen optimisation for rescue of metabolically challenged in vitro cortical brain tissue.IBRO reports · 2020Article
- Relationship between oxygen consumption and neuronal activity in a defined neural circuit.BMC biology · 2020Article
- A Metabolic Mechanism for Anaesthetic Suppression of Cortical Synaptic Function in Mouse Brain Slices-A Pilot Investigation.International journal of molecular sciences · 2020Article
- Adenosine AJournal of neurophysiology · 2019Article
- Quantitative in vivo imaging of neuronal glucose concentrations with a genetically encoded fluorescence lifetime sensor.Journal of neuroscience research · 2019Article
- Local oxygen homeostasis during various neuronal network activity states in the mouse hippocampus.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2019Article
- Lactate from astrocytes fuels learning-induced mRNA translation in excitatory and inhibitory neurons.Communications biology · 2019Article
- Sleep disruption elevates oxidative stress in parvalbumin-positive cells of the rat cerebral cortex.Sleep · 2019Article
- Metabolic modulation of neuronal gamma-band oscillations.Pflugers Archiv : European journal of physiology · 2018Article
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2 authors at 1 institution in 1 country.
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Abstract
The interactive vasculo-neuro-glial system controlling energy supply in the brain is absent in vitro where energy provision is determined by experimental conditions. Despite the fact that neuronal activity is extremely energy demanding, little has been reported on the state of energy metabolism in submerged brain slices. Without this information, the arbitrarily chosen oxygenation and metabolic provisions make questionable the efficient oxidative metabolism in slices. We show that in mouse hippocampal slices (postnatal day 19-44), evoked neuronal discharges, spontaneous network activity (initiated by 4-aminopyridine), and synaptic stimulation-induced NAD(P)H autofluorescence depend strongly on the oxygen availability. Only the rate of perfusion as high as ~15 ml/min (95% O(2)) provided appropriate oxygenation of a slice. Lower oxygenation resulted in the decrease of both local field potentials and spontaneous network activity as well as in significant modulation of short-term synaptic plasticity. The reduced oxygen supply considerably inhibited the oxidation phase of NAD(P)H signaling indicating that the changes in neuronal activity were paralleled by the decrease in aerobic energy metabolism. Interestingly, the dependence of neuronal activity on oxygen tension was clearly shifted toward considerably larger pO(2) values in slices when compared to in vivo conditions. With sufficient pO(2) provided by a high perfusion rate, partial substitution of glucose in ACSF for β-hydroxybutyrate, pyruvate, or lactate enhanced both oxidative metabolism and synaptic function. This suggests that the high pO(2) in brain slices is compulsory for maintaining oxidative metabolism, and glucose alone is not sufficient in fulfilling energy requirements during neuronal activity. Altogether, our results demonstrate that energy metabolism determines the functional state of neuronal network, highlighting the need for the adequate metabolic support to be insured in the in vitro experiments.
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