ArticleMolecular & cellular proteomics : MCP2012
Proteome analyses of hydrogen-producing hyperthermophilic archaeon Thermococcus onnurineus NA1 in different one-carbon substrate culture conditions.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 40 citations in OpenAlex.
- Engineering the hyperthermophilic archaeonApplied and environmental microbiology · 2025Article
- NADPApplied and environmental microbiology · 2023Article
- Discovery and remodeling of Vibrio natriegens as a microbial platform for efficient formic acid biorefinery.Nature communications · 2023Article
- Formate and hydrogen in hydrothermal vents and their use by extremely thermophilic methanogens and heterotrophs.Frontiers in microbiology · 2023Review
- HThe ISME journal · 2021Article
- Carbon monoxide-dependent transcriptional changes in a thermophilic, carbon monoxide-utilizing, hydrogen-evolving bacterium Calderihabitans maritimus KKC1 revealed by transcriptomic analysis.Extremophiles : life under extreme conditions · 2020Article
- Formate Utilization by the CrenarchaeonMicroorganisms · 2020Article
- Direct Electron Transfer between theApplied and environmental microbiology · 2020Article
- Metabolic Adaptation to Sulfur of HyperthermophilicInternational journal of molecular sciences · 2020Article
- Cross-Stress Adaptation in a Piezophilic and Hyperthermophilic Archaeon From Deep Sea Hydrothermal Vent.Frontiers in microbiology · 2020Article
- Characterization of thiosulfate reductase from Pyrobaculum aerophilum heterologously produced in Pyrococcus furiosus.Extremophiles : life under extreme conditions · 2020Article
- Proteomic analyses reveal that ginsenoside Rg3(Journal of ginseng research · 2020Article
- Genome-wide primary transcriptome analysis of HScientific reports · 2017Article
- Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1.Journal of microbiology (Seoul, Korea) · 2017Article
- High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii.Scientific reports · 2016Article
- Proteomic Insights into Sulfur Metabolism in the Hydrogen-Producing Hyperthermophilic Archaeon Thermococcus onnurineus NA1.International journal of molecular sciences · 2015Article
- Assessment of the Carbon Monoxide Metabolism of the Hyperthermophilic Sulfate-Reducing Archaeon Archaeoglobus fulgidus VC-16 by Comparative Transcriptome Analyses.Archaea (Vancouver, B.C.) · 2015Article
- Quantitative mass spectrometry reveals plasticity of metabolic networks in Mycobacterium smegmatis.Molecular & cellular proteomics : MCP · 2014Article
- Analysis of the complete genome of Fervidococcus fontis confirms the distinct phylogenetic position of the order Fervidicoccales and suggests its environmental function.Extremophiles : life under extreme conditions · 2014Article
- Engineering hydrogen gas production from formate in a hyperthermophile by heterologous production of an 18-subunit membrane-bound complex.The Journal of biological chemistry · 2014Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thermococcus onnurineus NA1, a sulfur-reducing hyperthermophilic archaeon, is capable of H(2)-producing growth, considered to be hydrogenogenic carboxydotrophy. Utilization of formate as a sole energy source has been well studied in T. onnurineus NA1. However, whether formate can be used as its carbon source remains unknown. To obtain a global view of the metabolic characteristics of H(2)-producing growth, a quantitative proteome analysis of T. onnurineus NA1 grown on formate, CO, and starch was performed by combining one-dimensional SDS-PAGE with nano UPLC-MS(E). A total of 587 proteins corresponding to 29.7% of the encoding genes were identified, and the major metabolic pathways (especially energy metabolism) were characterized at the protein level. Expression of glycolytic enzymes was common but more highly induced in starch-grown cells. In contrast, enzymes involved in key steps of the gluconeogenesis and pentose phosphate pathways were strongly up-regulated in formate-grown cells, suggesting that formate could be utilized as a carbon source by T. onnurineus NA1. In accordance with the genomic analysis, comprehensive proteomic analysis also revealed a number of hydrogenase clusters apparently associated with formate metabolism. On the other hand, CODH and CO-induced hydrogenases belonging to the Hyg4-II cluster, as well as sulfhydrogenase-I and Mbx, were prominently expressed during CO culture. Our data suggest that CO can be utilized as a sole energy source for H(2) production via an electron transport mechanism and that CO(2) produced from catabolism or CO oxidation by CODH and CO-induced hydrogenases may subsequently be assimilated into the organic carbon. Overall, proteomic comparison of formate- and CO-grown cells with starch-grown cells revealed that a single carbon compound, such as formate and CO, can be utilized as an efficient substrate to provide cellular carbon and/or energy by T. onnurineus NA1.
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