ArticleMolecular & cellular proteomics : MCP2012
Restricting fermentative potential by proteome remodeling: an adaptive strategy evidenced in Bacillus cereus.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
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- Evaluation of the Limit of Detection of Bacteria by Tandem Mass Spectrometry Proteotyping and Phylopeptidomics.Microorganisms · 2023Article
- Cysteine Proteome Reveals Response to Endogenous Oxidative Stress inInternational journal of molecular sciences · 2021Article
- Methionine Sulfoxide Reductases Contribute to Anaerobic Fermentative Metabolism inAntioxidants (Basel, Switzerland) · 2021Article
- Assessing the ratio of Bacillus spores and vegetative cells by shotgun proteomics.Environmental science and pollution research international · 2021Article
- Direct Meta-Analyses Reveal Unexpected Microbial Life in the Highly Radioactive Water of an Operating Nuclear Reactor Core.Microorganisms · 2020Article
- Quick microbial molecular phenotyping by differential shotgun proteomics.Environmental microbiology · 2020Review
- Titanium Dioxide Nanoparticles Alter the Cellular Phosphoproteome in A549 Cells.Nanomaterials (Basel, Switzerland) · 2020Article
- Advanced Proteomics as a Powerful Tool for Studying Toxins of Human Bacterial Pathogens.Toxins · 2019Review
- The PEG-responding desiccome of the alder microsymbiont Frankia alni.Scientific reports · 2018Article
- Proteogenomics data for decipheringData in brief · 2017Article
- Host Plant Compatibility Shapes the Proteogenome ofFrontiers in microbiology · 2017Article
- The species origin of the serum in the culture medium influences the in vitro toxicity of silica nanoparticles to HepG2 cells.PloS one · 2017Article
- Proteome data to explore the impact of pBClin15 on Bacillus cereus ATCC 14579.Data in brief · 2016Article
- Integrated analysis of shotgun proteomic data with PatternLab for proteomics 4.0.Nature protocols · 2016Article
- Adaptation inFrontiers in microbiology · 2016Review
- Understanding butanol tolerance and assimilation in Pseudomonas putida BIRD-1: an integrated omics approach.Microbial biotechnology · 2016Article
- High-throughput, quantitative assessment of the effects of low-dose silica nanoparticles on lung cells: grasping complex toxicity with a great depth of field.BMC genomics · 2015Article
- Proteomics identifies Bacillus cereus EntD as a pivotal protein for the production of numerous virulence factors.Frontiers in microbiology · 2015Article
- Time dynamics of the Bacillus cereus exoproteome are shaped by cellular oxidation.Frontiers in microbiology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenesis hinges on successful colonization of the gastrointestinal (GI) tract by pathogenic facultative anaerobes. The GI tract is a carbohydrate-limited environment with varying oxygen availability and oxidoreduction potential (ORP). How pathogenic bacteria are able to adapt and grow in these varying conditions remains a key fundamental question. Here, we designed a system biology-inspired approach to pinpoint the key regulators allowing Bacillus cereus to survive and grow efficiently under low ORP anoxic conditions mimicking those encountered in the intestinal lumen. We assessed the proteome components using high throughput nanoLC-MS/MS techniques, reconstituted the main metabolic circuits, constructed ΔohrA and ΔohrR mutants, and analyzed the impacts of ohrA and ohrR disruptions by a novel round of shotgun proteomics. Our study revealed that OhrR and OhrA are crucial to the successful adaptation of B. cereus to the GI tract environment. Specifically, we showed that B. cereus restricts its fermentative growth under low ORP anaerobiosis and sustains efficient aerobic respiratory metabolism, motility, and stress response via OhrRA-dependent proteome remodeling. Finally, our results introduced a new adaptive strategy where facultative anaerobes prefer to restrict their fermentative potential for a long term benefit.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.