ArticleActa crystallographica. Section F, Structural biology and crystallization communications2012
Measurement of the equilibrium relative humidity for common precipitant concentrations: facilitating controlled dehydration experiments.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed.
- Automated gradient equilibration of macromolecular crystals to new solution conditions.Acta crystallographica. Section F, Structural biology communications · 2025Article
- Multi-Temperature Crystallography of S-Adenosylmethionine Decarboxylase Observes Dynamic Loop Motions.Biomolecules · 2025Article
- Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization.Acta crystallographica. Section D, Structural biology · 2023Article
- A drug-discovery-oriented non-invasive protocol for protein crystal cryoprotection by dehydration, with application for crystallization screening.Journal of applied crystallography · 2022Article
- Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates.Journal of applied crystallography · 2021Article
- The low-cost Shifter microscope stage transforms the speed and robustness of protein crystal harvesting.Acta crystallographica. Section D, Structural biology · 2021Article
- Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron.IUCrJ · 2020Article
- Nonlinear Optical Investigation of Microbial Chromoproteins.Frontiers in plant science · 2020Article
- Controlled dehydration, structural flexibility and gadolinium MRI contrast compound binding in the human plasma glycoprotein afamin.Acta crystallographica. Section D, Structural biology · 2019Article
- On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies.IUCrJ · 2019Article
- Protein crystallization and initial neutron diffraction studies of the photosystem II subunit PsbO.Acta crystallographica. Section F, Structural biology communications · 2017Article
- High-speed fixed-target serial virus crystallography.Nature methods · 2017Article
- Article
- Raoult's law revisited: accurately predicting equilibrium relative humidity points for humidity control experiments.Journal of applied crystallography · 2017Article
- A generic protocol for protein crystal dehydration using the HC1b humidity controller.Acta crystallographica. Section D, Structural biology · 2016Article
- Crystal Dehydration in Membrane Protein Crystallography.Advances in experimental medicine and biology · 2016Article
- Preparation, Crystallization, and Structure Determination of Chromatin Enzyme/Nucleosome Complexes.Methods in enzymology · 2016Article
- An evaluation of adhesive sample holders for advanced crystallographic experiments.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Improved reproducibility of unit-cell parameters in macromolecular cryocrystallography by limiting dehydration during crystal mounting.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Measurement of the intrinsic variability within protein crystals: implications for sample-evaluation and data-collection strategies.Acta crystallographica. Section F, Structural biology communications · 2014Article
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4 authors.
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Abstract
The dehydration of crystals of macromolecules has long been known to have the potential to increase their diffraction quality. A number of methods exist to change the relative humidity that surrounds crystals, but for reproducible results, with complete characterization of the changes induced, a precise humidity-control device coupled with an X-ray source is required. The first step in these experiments is to define the relative humidity in equilibrium with the mother liquor of the system under study; this can often be quite time-consuming. In order to reduce the time spent on this stage of the experiment, the equilibrium relative humidity for a range of concentrations of the most commonly used precipitants has been measured. The relationship between the precipitant solution and equilibrium relative humidity is explained by Raoult's law for the equilibrium vapour pressure of water above a solution. The results also have implications for the choice of cryoprotectant and solutions used to dehydrate crystals. For the most commonly used precipitants (10-30% PEG 2000-8000), the starting point will be a relative humidity of 99.5%.
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