ArticleBMC genomics2012
Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed.
- First genome sequence of a European Alternaria brassicae isolate and genes involved in early development of alternaria leaf spot on Brassica juncea.Pest management science · 2026Article
- ESCRT: A Cellular Nexus of Plant Immunity and Pathogen Counter-Strategies.Molecular plant pathology · 2026Review
- The leucine-rich repeat receptor kinase QSK1 regulates PRR-RBOHD complexes targeted by the bacterial effector HopF2Pto.The Plant cell · 2024Article
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Barcode fusion genetics-protein-fragment complementation assay (BFG-PCA): tools and resources that expand the potential for binary protein interaction discovery.Nucleic acids research · 2022Article
- Unraveling Protein Interactions between the Temperate Virus Bam35 and ItsInternational journal of molecular sciences · 2021Article
- Revealing protein-protein interactions at the transcriptome scale by sequencing.Molecular cell · 2021Article
- Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor.PLoS computational biology · 2021Article
- Deciphering Molecular Host-Pathogen Interactions DuringFrontiers in plant science · 2021Article
- A holistic view on plant effector-triggered immunity presented as an iceberg model.Cellular and molecular life sciences : CMLS · 2020Review
- Keeping in Touch with Type-III Secretion System Effectors: Mass Spectrometry-Based Proteomics to Study Effector-Host Protein-Protein Interactions.International journal of molecular sciences · 2020Review
- High-resolution protein-protein interaction mapping using all-The Journal of biological chemistry · 2019Article
- IdentifyingG3 (Bethesda, Md.) · 2019Article
- Plant pathogen effector utilizes host susceptibility factor NRL1 to degrade the immune regulator SWAP70.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- An inhibitor of apoptosis (SfIAP) interacts with SQUAMOSA promoter-binding protein (SBP) transcription factors that exhibit pro-cell death characteristics.Plant direct · 2018Article
- A user-friendly platform for yeast two-hybrid library screening using next generation sequencing.PloS one · 2018Article
- The powdery mildew-resistant Arabidopsis mlo2 mlo6 mlo12 triple mutant displays altered infection phenotypes with diverse types of phytopathogens.Scientific reports · 2017Article
- Analysis of the ZAR1 Immune Complex Reveals Determinants for Immunity and Molecular Interactions.Plant physiology · 2017Article
- A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response.Scientific reports · 2017Article
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Authors and funding
9 authors.
Funding
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Abstract
backgroundIdentification of protein-protein interactions is a fundamental aspect of understanding protein function. A commonly used method for identifying protein interactions is the yeast two-hybrid system.
resultsHere we describe the application of next-generation sequencing to yeast two-hybrid interaction screens and develop Quantitative Interactor Screen Sequencing (QIS-Seq). QIS-Seq provides a quantitative measurement of enrichment for each interactor relative to its frequency in the library as well as its general stickiness (non-specific binding). The QIS-Seq approach is scalable and can be used with any yeast two-hybrid screen and with any next-generation sequencing platform. The quantitative nature of QIS-Seq data make it amenable to statistical evaluation, and importantly, facilitates the standardization of experimental design, data collection, and data analysis. We applied QIS-Seq to identify the Arabidopsis thaliana MLO2 protein as a target of the Pseudomonas syringae type III secreted effector protein HopZ2. We validate the interaction between HopZ2 and MLO2 in planta and show that the interaction is required for HopZ2-associated virulence.
conclusionsWe demonstrate that QIS-Seq is a high-throughput quantitative interactor screen and validate MLO2 as an interactor and novel virulence target of the P. syringae type III secreted effector HopZ2.
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