ArticleNucleic acid therapeutics2012
Degradation of nuclease-stabilized RNA oligonucleotides in Mycoplasma-contaminated cell culture media.
Article in Nucleic acid therapeutics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Aptamers in Osteosarcoma: New Paths for Diagnosis and Treatment.Anti-cancer agents in medicinal chemistry · 2026Review
- Aptamer-based approaches in leukemia: a paradigm shift in targeted therapy.Clinical and experimental medicine · 2025Review
- Aptamers: precision tools for diagnosing and treating infectious diseases.Frontiers in cellular and infection microbiology · 2024Review
- Selection of a novel cell-internalizing RNA aptamer specific for CD22 antigen in B cell acute lymphoblastic leukemia.Molecular therapy. Nucleic acids · 2023Article
- Chemically Modified Poly(A) Analogs Targeting PABP: Structure Activity Relationship and Translation Inhibitory Properties.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Challenges in the use of nanostructures as carriers of nucleic acids in clinical practice.Einstein (Sao Paulo, Brazil) · 2022Review
- Nano optical and electrochemical sensors and biosensors for detection of narrow therapeutic index drugs.Mikrochimica acta · 2021Review
- Discovery and Proof-of-Concept Study of Nuclease Activity as a Novel Biomarker for Breast Cancer Tumors.Cancers · 2021Article
- Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance.Biosensors & bioelectronics · 2020Article
- Mycoplasma contamination affects cell characteristics and decreases the sensitivity of BV2 microglia to LPS stimulation.Cytotechnology · 2019Article
- Aptamer Therapeutics in Cancer: Current and Future.Cancers · 2018Review
- Inhibition of Poly(A)-binding protein with a synthetic RNA mimic reduces pain sensitization in mice.Nature communications · 2018Article
- Rapid and Sensitive Detection of Breast Cancer Cells in Patient Blood with Nuclease-Activated Probe Technology.Molecular therapy. Nucleic acids · 2017Article
- Method for Confirming Cytoplasmic Delivery of RNA Aptamers.Methods in molecular biology (Clifton, N.J.) · 2016Article
- The effects of mycoplasma contamination upon the ability to form bioengineered 3D kidney cysts.PloS one · 2015Article
- Cell-internalization SELEX: method for identifying cell-internalizing RNA aptamers for delivering siRNAs to target cells.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Noninvasive imaging of Staphylococcus aureus infections with a nuclease-activated probe.Nature medicine · 2014Article
- Article
- Aptamers: problems, solutions and prospects.Acta naturae · 2013Article
- Methods for Evaluating Cell-Specific, Cell-Internalizing RNA Aptamers.Pharmaceuticals (Basel, Switzerland) · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Artificial RNA reagents such as small interfering RNAs (siRNAs) and aptamers often must be chemically modified for optimal effectiveness in environments that include ribonucleases. Mycoplasmas are common bacterial contaminants of mammalian cell cultures that are known to produce ribonucleases. Here we describe the rapid degradation of nuclease-stabilized RNA oligonucleotides in a human embryonic kidney 293 (HEK) cell culture contaminated with Mycoplasma fermentans, a common species of mycoplasma. RNA with 2'-fluoro- or 2'-O-methyl- modified pyrimidines was readily degraded in conditioned media from this culture, but was stable in conditioned media from uncontaminated HEK cells. RNA completely modified with 2'-O-methyls was not degraded in the mycoplasma-contaminated media. RNA zymogram analysis of conditioned culture media and material centrifuged from the media revealed several distinct protein bands (ranging from 30 to 68 kDa) capable of degrading RNA with 2'-fluoro- or 2'-O-methyl-modified pyrimidines. Finally, the mycoplasma-associated nuclease was detected in material centrifuged from the contaminated culture supernatants in as little as 15 minutes with an RNA oligo-containing 2'-O-methyl-modified pyrimidines and labeled with a 5'-fluorescein amidite (FAM) and 3'-quencher. These results suggest that mycoplasma contamination may be a critical confounding variable for cell culture experiments involving RNA-based reagents, with particular relevance for applications involving naked RNA (e.g., aptamer-siRNA chimeras).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.