ArticleThe Journal of biological chemistry2012
Identification of N-acetyltaurine as a novel metabolite of ethanol through metabolomics-guided biochemical analysis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- Tissue-dependent enzymatic control of N-acetyl-β-alanine by PTER.The Journal of biological chemistry · 2026Article
- A small molecule PTER-selective inhibitor reduces food intake and body weight.bioRxiv : the preprint server for biology · 2026Article
- Reactions ofEnvironmental science & technology · 2025Article
- Novel Data-Driven Mechanistic Modeling of Untargeted Metabolome Data Reveals Feed Component Effects in CHO Cell Bioprocess Using Column Generation-Based EFMs.Biotechnology journal · 2025Article
- Article
- Characterization of UrinaryMetabolites · 2024Article
- A PTER-dependent pathway of taurine metabolism linked to energy balance.bioRxiv : the preprint server for biology · 2024Article
- Taurine deficiency as a driver of aging.Science (New York, N.Y.) · 2023Article
- Article
- Article
- Protective role of taurine against oxidative stress (Review).Molecular medicine reports · 2021Review
- Salivary metabolites associated with a 5-year tooth loss identified in a population-based setting.BMC medicine · 2021Article
- Disease-associated gut microbiome and metabolome changes in patients with chronic obstructive pulmonary disease.Nature communications · 2020Article
- Metabolomic and Lipidomic Biomarkers for Premalignant Liver Disease Diagnosis and Therapy.Metabolites · 2020Review
- Glutathione deficiency-elicited reprogramming of hepatic metabolism protects against alcohol-induced steatosis.Free radical biology & medicine · 2019Article
- Study of Fecal and Urinary Metabolite Perturbations Induced by Chronic Ethanol Treatment in Mice by UHPLC-MS/MS Targeted Profiling.Metabolites · 2019Article
- Transgenic mice and metabolomics for study of hepatic xenobiotic metabolism and toxicity.Expert opinion on drug metabolism & toxicology · 2015Review
- Molecular mechanisms of ethanol-associated oro-esophageal squamous cell carcinoma.Cancer letters · 2015Review
- Role of farnesoid X receptor and bile acids in alcoholic liver disease.Acta pharmaceutica Sinica. B · 2015Review
- Alterations of the gut microbiome and metabolome in alcoholic liver disease.World journal of gastrointestinal pathophysiology · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The influence of ethanol on the small molecule metabolome and the role of CYP2E1 in ethanol-induced hepatotoxicity were investigated using liquid chromatography-mass spectrometry (LC-MS)-based metabolomics platform and Cyp2e1-null mouse model. Histological and biochemical examinations of ethanol-exposed mice indicated that the Cyp2e1-null mice were more resistant to ethanol-induced hepatic steatosis and transaminase leakage than the wild-type mice, suggesting CYP2E1 contributes to ethanol-induced toxicity. Metabolomic analysis of urinary metabolites revealed time- and dose-dependent changes in the chemical composition of urine. Along with ethyl glucuronide and ethyl sulfate, N-acetyltaurine (NAT) was identified as a urinary metabolite that is highly responsive to ethanol exposure and is correlated with the presence of CYP2E1. Subsequent stable isotope labeling analysis using deuterated ethanol determined that NAT is a novel metabolite of ethanol. Among three possible substrates of NAT biosynthesis (taurine, acetyl-CoA, and acetate), the level of taurine was significantly reduced, whereas the levels of acetyl-CoA and acetate were dramatically increased after ethanol exposure. In vitro incubation assays suggested that acetate is the main precursor of NAT, which was further confirmed by the stable isotope labeling analysis using deuterated acetate. The incubations of tissues and cellular fractions with taurine and acetate indicated that the kidney has the highest NAT synthase activity among the tested organs, whereas the cytosol is the main site of NAT biosynthesis inside the cell. Overall, the combination of biochemical and metabolomic analysis revealed NAT is a novel metabolite of ethanol and a potential biomarker of hyperacetatemia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.